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SCR131 Human iPSC Derived Neural Progenitor Kit

Human iPSC Derived Neural Progenitor Kit : SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Key Applications: Cell Differentiation, Stem Cell Culture, Cell Culture, Neurotoxicity, Neuroscience
SCR131
1x10⁶ cells and 500ml optimized expansion medium/Kit  
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      Replacement Information

      Key Spec Table

      Key Applications
      Diff, Cult, CULT, Neurotoxicity, Neuroscience
      Description
      Catalogue NumberSCR131
      DescriptionHuman iPSC Derived Neural Progenitor Kit
      Alternate Names
      • Neural Stem Cell
      • IPS NSC
      Background InformationEMD Millipore’s Human iPSC Derived Neural Progenitor Kit contains human neural progenitor cells (NPCs) along with optimized medium for their expansion. Human neural progenitor cells (NPCs) are derived from induced pluripotent stem (iPS) cells generated using STEMCCA Cre-Excisable Constitutive Polycistronic (OKSM) lentivirus. The viral transgenes have been removed using cell permeable TAT-Cre recombinase (Cat. No. SCR508) with excision confirmed by quantitative PCR of genomic DNA extracted from human iPSC cells.

      These hiPSC-derived NPCs proliferate as an adherent cell monolayer with greater than 80% expression of the appropriate neural stem cell markers, including Nestin and Sox-2. Cells are provided at passage 3 and can be expanded for a further three to five passages with ENStem-A Neural Expansion Medium (Cat. No. SCM004). Human NPCs can be further differentiated to terminal neurons by the use of the Human ES/iPS Neuronal Differentiation Medium (Cat. No. SCM111) or ENStem-A Neuronal Differentiation Medium (Cat. No. SCM017). Differentiation leads to greater than 80-90% of the cells expressing mostly neuronal markers, bIII-tubulin and MAP2.

      Human iPSC-derived NPCs can be used for a variety of research applications including studies of neurotoxicity, co-culture applications and screening for molecules that induce or inhibit preferential differentiation to mature neurons.
      References
      Product Information
      Components
      • 1) 1x106 viable Human iPSC Derived Neural Progenitors: (Catalog No. SCC035). Store in liquid nitrogen.
      • 2) 500 mL ENStem-A Neural Expansion Medium (Catalog No. SCM004). Store at -20c.
      Quality LevelMQ100
      Applications
      Key Applications
      • Cell Differentiation
      • Stem Cell Culture
      • Cell Culture
      • Neurotoxicity
      • Neuroscience
      Biological Information
      Stem Cell Type
      • Neural Stem Cells
      • Induced Pluripotent Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsENStem-ATM Neural Expansion Medium (Cat. No. SCM004) should be stored at -20C upon receiving.

      Human iPSC-Derived Neural Progenitors Cells (Cat. No. SCC035) should be stored in liquid nitrogen immediately after receiving. Temperature fluctuations will impair the recovery of healthy cells. Do not thaw the human cells until you are ready to do the assay of choice.
      Packaging Information
      Material Size1x10⁶ cells and 500ml optimized expansion medium/Kit
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      SCR131 04053252910395