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324699 Elastase Substrate IV, Colorimetric - Calbiochem

A specific substrate for pancreatic elastase.

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324699
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324699-5MG
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      		 Kst.-Ampulle 5 mg
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      Description
      OverviewA specific substrate for pancreatic elastase (km = 100 µM; kcat/ Km = 35,000 M-1 s-1 for rat pancreatic elastase, Km = 30 µM; kcat/Km = 35,000 M-1 s-1 for porcine). Cleavage of substrate can be monitored at ~405 nm.
      Catalogue Number324699
      Brand Family Calbiochem®
      References
      ReferencesLargman, L. 1983. Biochemistry 22, 3763.
      Del Mar, E.G., et al. 1980. Biochemistry 19, 468.
      Product Information
      ATP CompetitiveN
      FormWhite to off-white lyopilized solid
      Formulation45 mg D-mannitol and 5 mg substrate. Sold on the basis of substrate content.
      Hill FormulaC₂₅H₃₄N₆O₉
      Chemical formulaC₂₅H₃₄N₆O₉
      ReversibleN
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetA specific substrate for pancreatic elastase
      Primary Target IC<sub>50</sub>km = 100 µM; kcat/ Km = 35,000 M-1 s-1 for rat pancreatic elastase; Km = 30 µM; kcat/Km = 35,000 M-1 s-1 for porcine
      Purity≥95% by HPLC
      Physicochemical Information
      Cell permeableN
      Peptide SequenceSuc-Ala-Ala-Pro-Abu-pNA (Abu = L-α-Aminobutyric Acid)
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Moisture Protect from moisture
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 3 months at 4°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      324699-5MG 04055977215915

      Documentation

      Elastase Substrate IV, Colorimetric - Calbiochem SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Elastase Substrate IV, Colorimetric - Calbiochem Analysenzertifikate

      TitelChargennummer
      324699

      Literatur

      Übersicht
      Largman, L. 1983. Biochemistry 22, 3763.
      Del Mar, E.G., et al. 1980. Biochemistry 19, 468.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-June-2008 RFH
      DescriptionA specific substrate for pancreatic elastase (Km = 100 µM; Kcat/Km = 35,000 s-1 M-1 for rat pancreatic elastase; Km = 30 µM; Kcat/Km = 351,000 s-1 M-1 for porcine pancreatic elastase). Cleavage of substrate can be monitored at ~405 nm.

      Suggested protocol: see Largman, C. (1983)
      FormWhite to off-white lyopilized solid
      Formulation45 mg D-mannitol and 5 mg substrate. Sold on the basis of substrate content.
      Chemical formulaC₂₅H₃₄N₆O₉
      Peptide SequenceSuc-Ala-Ala-Pro-Abu-pNA (Abu = L-α-Aminobutyric Acid)
      Purity≥95% by HPLC
      SolubilityEthanol (1 mg/ml) or H₂O
      Storage Protect from moisture
      Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 3 months at 4°C.
      Toxicity Standard Handling
      ReferencesLargman, L. 1983. Biochemistry 22, 3763.
      Del Mar, E.G., et al. 1980. Biochemistry 19, 468.