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Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
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Gewähltes Kit
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96-Well Plate
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Platzsparende Option Kunden, die mehrere Kits kaufen, können ihre Multiplex-Assaykomponenten in Kunststoffbeuteln anstelle von Packungen erhalten, um eine kompaktere Lagerung zu ermöglichen.
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Pigment Epithelium-Derived Factor (PEDF) was first identified as a factor secreted by primary retinal pigment epithelial (RPE) cells that promotes the survival of neurons in vitro and in vivo. Molecular characterization of PEDF shows it to be a 50 kDa member of the serpin family of protease inhibitors1. A second mode of action of PEDF was identified with the demonstration that PEDF induces apoptosis of microvascular endothelial cells, a property that maintains the avascular nature of the retina2. As a number of ophthalmologic disorders are characterized by dysregulation of the retinal innervation and microvasculature, PEDF serves as an important regulator of human eye disease3. In addition, PEDF appears to have multifunctional antitumor activity in a neuroblastoma model, as Schwann cell-derived PEDF induces a differentiated, less malignant phenotype in neuroblastoma cells, promotes further growth and survival of Schwann cells, and inhibits angiogenesis4.
ChemiKine™ Pigment Epithelium-Derived Factor (PEDF) kit is a sandwich stripwell-type enzyme immunoassay (EIA), which measures PEDF. The kit will measure PEDF of vitreous fluid of human origin.
Test Principle
With the ChemiKine™ PEDF assay system, a mouse monoclonal antibody generated against human PEDF is coated onto a microplate and is used to capture PEDF from a sample. A second, biotinylated PEDF-specific mouse monoclonal antibody detects the captured PEDF. Peroxidase-labeled streptavidin detects the biotinylated mouse monoclonal antibody. After addition of the substrate and stop solution the amount of PEDF is determined. The standard curve demonstrates a direct relationship between Optical Density (OD) and PEDF concentration: i.e., the higher the OD the higher the PEDF concentration in the sample.
Alternate Names
PEDF
Materials Required but Not Delivered
1. Multi-channel or repeating pipettes
2. 37ºC incubator
3. Plate sealers
4. Pipettors & tips capable of accurately measuring 10-1000 mL
5. Graduated serological pipettes
6. 96-well microplate reader with 450 nm filter
7. Graph paper for manual plotting of data
8. Polypropylene test tubes for standard and sample dilutions
9. Mechanical vortex
10. One 1 or 2 liter container
11. Urea
References
Product Information
Components
PEDF ELISA Plate: (Part No. 61597) One 96-Well Immunoplate Pre-coated with Mouse anti-Human PEDF Monoclonal Antibody, sealed in a foil pouch.
Wash Buffer Concentrate: (Part No. 60245) One 100 mL (10X) bottle of Concentrate.
Assay Diluent: (Part No. 60240) One 60 mL bottle (Ready to Use).
PEDF Standard (Human): (Part No. 61600) One vial containing 250 ng recombinant full length human PEDF (Lyophilized).
This ChemiKine Pigment Epithelium Derived Factor, Sandwich ELISA is used to measure & quantify PEDF levels in Neuroscience research.
Key Applications
ELISA
Application Notes
ChemiKine™ PEDF kit is designed to measure the amount of PEDF in cell culture supernatants, tissue homogenates and biological fluid (vitreous, serum, plasma, and serum-free) samples of human origin. There are enough reagents included in this kit for one 96-well immunoassay plate. Running duplicate wells for samples and standards is recommended.
For Research Use Only. Not for use in diagnostic procedures.
FUNCTION: SwissProt: P36955 # Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity. SIZE: 418 amino acids; 46342 Da SUBCELLULAR LOCATION: Secreted. Melanosome. Note=Enriched in stage I melanosomes. TISSUE SPECIFICITY: Retinal pigment epithelial cells and blood plasma. DEVELOPMENTAL STAGE: Expressed in quiescent cells. DOMAIN: SwissProt: P36955 The N-terminal (AA 44-121) exhibits neurite outgrowth- inducing activity. The C-terminal exposed loop (AA 382-418) is essential for serpin activity. PTM: The N-terminus is blocked. SIMILARITY: Belongs to the serpin family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
For research use only. Not for use in diagnostic procedures.
Storage and Shipping Information
Storage Conditions
Maintain the unopened kit at 2-8°C until expiration date indicated on the label. After opening the kit maintain each component at 2-8°C until expiration date indicated on the label. After reconstitution, maintain the PEDF Standard at -20ºC for up to 30 days. The standard may be frozen and thawed three times without loss of immunoreactivity. Do not store reconstituted standard at 4ºC, as loss of immunoreactivity will result.
Precautions
· Wash Buffer and Assay Diluent contain thimerosal. Thimerosal is highly toxic by inhalation, contact with skin or if swallowed. Thimerosal is a possible mutagen and should be handled accordingly.
· The instructions provided have been designed to optimize the kit's performance. Deviation from the instructions may result in suboptimal performance of the kit and the failure to produce accurate data.
Derivation and comparative assessment of retinal pigment epithelium from human embryonic stem cells using transcriptomics Klimanskaya, I. et al. Cloning and Stem Cells, 6:217-245 (2004)
2004
Suppression of corneal neovascularization by PEDF release from human amniotic membranes. Shao, Chunkui, et al. Invest. Ophthalmol. Vis. Sci., 45: 1758-62 (2004)
2004
Effect of over-expression of pigment epithelium derived factor (PEDF) on developing retinal vasculature in the mouse Wong, Wai T, et al Mol Vis, 10:837-44 (2004)
2004
Acute Injury and Chronic Neurodegeneration Antibodies, Immunoassays, & Histological Stains
Millipore offers a range of products for neurodegeneration research: detection & staining of dying neurons. See below for products for products based on the expertise of Upstate & Chemicon. Weitere Informationen >>
