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Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
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96-Well Plate
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
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Anti-phospho-TBC1D1 (Ser237), Cat. No. 07-2268, is a rabbit polyclonal antibody that detects TBC1D1 phosphorylated on serine 237 and is tested for use in Peptide Inhibition Assay and Western Blotting.
More>>Anti-phospho-TBC1D1 (Ser237), Cat. No. 07-2268, is a rabbit polyclonal antibody that detects TBC1D1 phosphorylated on serine 237 and is tested for use in Peptide Inhibition Assay and Western Blotting. Less<<
Anti-phospho-TBC1D1 Antibody (Ser237): SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1
Background Information
TBC1 domain family member 1 (UniProt: Q86TI0; also known as TBC1D1) is encoded by the TBC1D1 gene (Gene ID: 23216) in human. TBC1D1 is a member of the TBC1 Rab-GTPase family of nuclear proteins that is highly expressed in skeletal muscle. Its major action is to regulate exercise-mediated glucose uptake into muscle. It is involved in the trafficking and translocation of GLUT4-containing vesicles and insulin-stimulated glucose uptake into cells. Insulin and muscle contraction are reported to increase TBC1D1 phosphorylation on phospho-Akt substrate motifs. Insulin-stimulated phosphorylation by AKT is shown to stimulate GLUT4 translocation. Following muscle exercise, phosphorylation of TBC1D1 at serine 237 by AMPK and 14-3-3 protein binding capacity are significantly increased. Knockdown of TBC1D1 in C2C12 muscle cells is shown to increase fatty acid oxidation and reduce muscle levels of GLUT4. Although TBC1D1 knockout mice do not exhibit any disruption in their whole-body insulin action, they display impaired exercise-regulated metabolism. A mutation in TBC1D1 (R125W) has been linked to obesity in human. (Ref.: Stockli, J., et al. (2015). Diabetes. 64(6); 1914-1922; An, D., et al. (2010). Diabetes. 59(6); 1358-1365; Frosig, C., et al. (2010). J. Physiol. 588(22); 4539-4548).
References
Product Information
Format
Affinity Purified
Control
Lambda phosphatase treated and untreated HEK293 cell lysates
Presentation
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide
Anti-phospho-TBC1D1 (Ser237), Cat. No. 07-2268, is a rabbit polyclonal antibody that detects TBC1D1 phosphorylated on serine 237 and is tested for use in Peptide Inhibition Assay and Western Blotting.
Key Applications
Western Blotting
Peptide Inhibition Assay
Application Notes
Tested Applications
Peptide Inhibition Assay: Target band detection in HEK293 cell lysate was prevented by preblocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
Immunogen
KLH-conjugated linear peptide corresponding to 13 amino acids surrounding phosphoserine 237 of human TBC1 domain family member 1 (TBC1D1).
Epitope
Phosphorylated Ser237
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
This rabbit polyclonal antibody detects TBC1D1 phosphorylated on serine 237.
Species Reactivity
Human
Species Reactivity Note
Human. Predicted to react with Bovine, Monkey, Chimpanzee, Mouse, Rat based on 100% sequence homology.
TBC1D1 is the founding member of a family of proteins sharing a 180- to 200-amino acid TBC domain presumed to have a role in regulating cell growth and differentiation. These proteins share significant homology with TRE2 (USP6; MIM 604334), yeast Bub2, and CDC16 (MIM 603461) (White et al., 2000 [PubMed 10965142]).[supplied by OMIM].
FUNCTION: May act as a GTPase-activating protein for Rab family protein(s). May play a role in the cell cycle and differentiation of various tissues (By similarity).
SUBCELLULAR LOCATION: Nucleus (By similarity).
SEQUENCE SIMILARITIES: Contains 1 PID domain.
Contains 1 Rab-GAP TBC domain.
SEQUENCE CAUTION: The sequence BAB55057.1 differs from that shown. Reason: Erroneous initiation.
Molecular Weight
~158 kDa observed; 133.08 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in lysate from HEK293 cells treated with phorbol-12-myristate-13-acetate (PMA).
Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected TBC1D1 lysate from HEK293 cells treated with phorbol-12-myristate-13-acetate (PMA). However, the detection was inhibited blots pre-incubated with protein phosphatase.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
