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04-1017 Anti-phospho-MEK1/2 Ser218/Ser222) Antibody, clone E237, rabbit monoclonal

Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.

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Research Category: Signaling Research Sub-Category: MAP Kinases Gene Symbol: EC 2.7.12.2 , MAPKK1, MEK1, MKK1, PRKMK1 UniProt Number: Q02750
04-1017
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICC, IH(P), IPRbUnpurifiedMonoclonal Antibody
      Description
      Catalogue Number04-1017
      DescriptionAnti-phospho-MEK1/2 Ser218/Ser222) Antibody, clone E237, rabbit monoclonal
      Alternate Names
      • MAP kinase kinase 1
      • MAPK/ERK kinase 1
      • MAPKK 1
      • mitogen-activated protein kinase kinase 1
      • protein kinase, mitogen-activated
      • kinase 1 (MAP kinase kinase 1)
      • ERK activator kinase 1
      Background InformationMEK1/2, also known as MAP2K1, are mitogen activated protein kinases and are involved in cell growth and differentiation. MEK is activated by a variety of growth factors and cytokines resulting in phosphorylation at Ser217/218 and Ser221/222. Diseases associated with MEK1/2 is cardio-facio- cutaneous syndrome (CFC), which is an autosomal dominant trait.
      References
      Product Information
      FormatUnpurified
      Control
      • Untreated and EGF-treated serum-starved A431 cell lysate
      PresentationRabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      Application NotesImmunohistochemistry Analysis: A 1:50-100 dilution from a previous lot detected MEK1/2 in skin carcinoma tissue.
      Immunocytochemistry Analysis: A 1:50-100 dilution from a previous lot was used in IC.
      Immunoprecipitation Analysis: A 1:30 dilution from a previous lot was used in IP.
      Biological Information
      ImmunogenPhospho-synthetic peptide corresponding to residues surrounding Ser218 and Ser222 of human MEK1/2.
      EpitopePhosphorylated Ser218/Ser222
      CloneE237
      HostRabbit
      SpecificityThe antibody only detects MEK1/2 phosphorylated on Ser218 and Ser222.
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EC 2.7.12.2
      • MAPKK1
      • MEK1
      • MKK1
      • PRKMK1
      Modifications
      • Phosphorylation
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
      CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
      ENZYME REGULATION: Activated by phosphorylation.
      SUBUNIT STRUCTURE: Interacts with MORG1 By similarity. Interacts with Yersinia yopJ.
      PTM: Phosphorylation on Ser/Thr by MAP kinase kinase kinases (RAF or MEKK1) regulates positively the kinase activity.
      Acetylation by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway.
      INVOLVEMENT IN DISEASE: Defects in MAP2K1 are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
      Contains 1 protein kinase domain.
      Molecular Weight~45 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in untreated and EGF-treated serum-starved A431 cell lysates.
      Western Blot Analysis: A 1:500-1,000 dilution of this antibody was used to detect MEK1/2 in untreated and EGF-treated serum-starved A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      04-1017 04053252625893

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      Kategorien

      Life Science Research > Antibodies and Assays > Primary Antibodies