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96-Well Plate
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48-602MAG
Buffer Detection Kit for Magnetic Beads
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Anti-monomethyl-Histone H4 (Lys20) Antibody is a rabbit polyclonal antibody for detection of monomethyl-Histone H4 (Lys20) also known as H4K20me1, Histone H4 (mono methyl K20) & has been validated in WB, Mplex, ICC.
More>>Anti-monomethyl-Histone H4 (Lys20) Antibody is a rabbit polyclonal antibody for detection of monomethyl-Histone H4 (Lys20) also known as H4K20me1, Histone H4 (mono methyl K20) & has been validated in WB, Mplex, ICC. Less<<
Anti-monomethyl-Histone H4 (Lys20) Antibody: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Anti-monomethyl-Histone H4 (Lys20) Antibody is a rabbit polyclonal antibody for detection of monomethyl-Histone H4 (Lys20) also known as H4K20me1, Histone H4 (mono methyl K20) & has been validated in WB, Mplex, ICC.
Key Applications
Western Blotting
Multiplexing
Immunocytochemistry
Application Notes
Additional Referenced Research Applications: Immunocytochemistry (cells)
Biological Information
Immunogen
KLH-conjugated, branched synthetic peptide corresponding to amino acids 15-25 (AKRHR[me1K]VLRDN) of human Histone H4
Host
Rabbit
Specificity
monomethylated Histone H4 (Lys20)
Isotype
IgG
Species Reactivity
Human
Species Reactivity Note
Broad species cross-reactivity expected due to sequence conservation
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6.
FUNCTION: SwissProt: P62805 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. SIZE: 103 amino acids; 11367 Da SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. SUBCELLULAR LOCATION: Nucleus. PTM: Symmetric dimethylation on Arg-4 by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage (By similarity). & Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. SIMILARITY: SwissProt: P62805 ## Belongs to the histone H4 family.
Molecular Weight
10kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Routinely evaluated by immunoblot
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Regulation of Gγ-globin gene by ATF2 and its associated proteins through the cAMP-response element. Liu, L; Karmakar, S; Dhar, R; Mahajan, M; Choudhury, A; Weissman, S; Pace, BS PloS one
8
e78253
2013
The upstream Gγ-globin cAMP-response element (G-CRE) plays an important role in regulating Gγ-globin expression through binding of ATF2 and its DNA-binding partners defined in this study. ATF2 knockdown resulted in a significant reduction of γ-globin expression accompanied by decreased ATF2 binding to the G-CRE. By contrast, stable ATF2 expression in K562 cells increased γ-globin transcription which was reduced by ATF2 knockdown. Moreover, a similar effect of ATF2 on γ-globin expression was observed in primary erythroid progenitors. To understand the role of ATF2 in γ-globin expression, chromatographically purified G-CRE/ATF2-interacting proteins were subjected to mass spectrometry analysis; major binding partners included CREB1, cJun, Brg1, and histone deacetylases among others. Immunoprecipitation assays demonstrated interaction of these proteins with ATF2 and in vivo GCRE binding in CD34(+) cells undergoing erythroid differentiation which was correlated with γ-globin expression during development. These results suggest synergism between developmental stage-specific recruitments of the ATF2 protein complex and expression of γ-globin during erythropoiesis. Microarray studies in K562 cells support ATF2 plays diverse roles in hematopoiesis and chromatin remodeling.
Millipore’s Histone H4 antibodies demonstrate specificity against linker histone H4. See below for H4 Antibodies, ChipAb+ Primer sets, and peptides, based on the expertise of Upstate & Chemicon. Weitere Informationen >>
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