Wenn Sie das Fenster schließen, wird Ihre Konfiguration nicht gespeichert, es sei denn, Sie haben Ihren Artikel in die Bestellung aufgenommen oder zu Ihren Favoriten hinzugefügt.
Klicken Sie auf OK, um das MILLIPLEX® MAP-Tool zu schließen oder auf Abbrechen, um zu Ihrer Auswahl zurückzukehren.
Wählen Sie konfigurierbare Panels & Premixed-Kits - ODER - Kits für die zelluläre Signaltransduktion & MAPmates™
Konfigurieren Sie Ihre MILLIPLEX® MAP-Kits und lassen sich den Preis anzeigen.
Konfigurierbare Panels & Premixed-Kits
Unser breites Angebot enthält Multiplex-Panels, für die Sie die Analyten auswählen können, die am besten für Ihre Anwendung geeignet sind. Unter einem separaten Register können Sie das Premixed-Cytokin-Format oder ein Singleplex-Kit wählen.
Kits für die zelluläre Signaltransduktion & MAPmates™
Wählen Sie gebrauchsfertige Kits zur Erforschung gesamter Signalwege oder Prozesse. Oder konfigurieren Sie Ihre eigenen Kits mit Singleplex MAPmates™.
Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
.
Bestellnummer
Bestellinformationen
St./Pkg.
Liste
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Wählen Sie bitte Spezies, Panelart, Kit oder Probenart
Um Ihr MILLIPLEX® MAP-Kit zu konfigurieren, wählen Sie zunächst eine Spezies, eine Panelart und/oder ein Kit.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Catalogue Number
Ordering Description
Qty/Pack
List
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Spezies
Panelart
Gewähltes Kit
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
96-Well Plate
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
Weitere Reagenzien hinzufügen (MAPmates erfordern die Verwendung eines Puffer- und Detektionskits)
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Platzsparende Option Kunden, die mehrere Kits kaufen, können ihre Multiplex-Assaykomponenten in Kunststoffbeuteln anstelle von Packungen erhalten, um eine kompaktere Lagerung zu ermöglichen.
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Das Produkt wurde in Ihre Bestellung aufgenommen
Sie können nun ein weiteres Kit konfigurieren, ein Premixed-Kit wählen, zur Kasse gehen oder das Bestell-Tool schließen.
MAB4234-C
Sigma-AldrichAnti-Wilms' Tumor Antibody, NT clone 6F-H2, Ascites Free
This Anti-Wilms' Tumor Antibody, NT clone 6F-H2, Ascites Free is validated for use in Immunocytochemistry, Immunoprecipitation, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting for the detection of Wilms' tumor protein.
More>>This Anti-Wilms' Tumor Antibody, NT clone 6F-H2, Ascites Free is validated for use in Immunocytochemistry, Immunoprecipitation, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting for the detection of Wilms' tumor protein. Less<<
SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Anti-Wilms' Tumor Antibody, NT clone 6F-H2, Ascites Free
Alternate Names
Wilms tumor protein
WT33
Background Information
Wilms tumor protein (UniProt P19544; also known as WT33) is encoded by the WT1 (also known as DDS, FS, MEACHS, MESOM, NPHS4, WAGR) gene (Gene ID 7490) in human. The Wilms’ tumor gene WT1 was originally identified in the childhood kidney cancer Wilms’ tumor. The N-terminal region of WT1 protein contains a proline-rich region (a.a. 27-83) involved in transcriptional regulation, self-association, and RNA recognition, while its C-terminal region contains four zinc fingers (a.a 323-347, 353-377, 383-405, 414-438) that mediate DNA and RNA binding. The zinc finger domain of WT1 can bind to GC-rich sequences, such as the EGR-1 consensus sequence (5’-GCG(T/G)GGGCG-3’), the WTE motif (5'-GCGTGGGAGT-3'), or (TCC)n motif. Many genes responsible for cell growth and apoptosis, such as Bcl-2, Bcl-xL, BFL1, and c-myc, have been identified as downstream targets of WT1. There are four major alternatively spliced WT1 isoforms resulting from splicing at either or both of exon 5 (17AA) and exon 9 (KTS). All four major WT1 isoforms are overexpressed in leukemia and solid tumors and play oncogenic roles such as inhibition of apoptosis, and promotion of cell proliferation, migration and invasion.
References
Product Information
Format
Purified
Presentation
Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
This Anti-Wilms' Tumor Antibody, NT clone 6F-H2, Ascites Free is validated for use in Immunocytochemistry, Immunoprecipitation, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting for the detection of Wilms' tumor protein.
Key Applications
Immunocytochemistry
Immunofluorescence
Immunohistochemistry (Paraffin)
Immunoprecipitation
Western Blotting
Application Notes
Immunohistochemistry Analysis: An 1:250 dilution from a representative lot detected Wilms tumor protein in human kidney tissue.
Immunoprecipitation Analysis: A representative lot co-immunoprecipitated CRE-binding protein/CBP together with Wilms tumor protein WT1 from the lysate of a T-SV40 immortalized human glomerular epithelial cell (HGEC) line (Drossopoulou, G.I., et al. (2009). Am. J. Physiol. Renal Physiol. 297(3):F594-F603).
Western Blotting Analysis: A representative lot detected Wilms tumor protein WT1 in the CRE-binding protein/CBP immunoprecipitate obtained from the lysate of a T-SV40 immortalized human glomerular epithelial cell (HGEC) line (Drossopoulou, G.I., et al. (2009). Am. J. Physiol. Renal Physiol. 297(3):F594-F603).
Western Blotting Analysis: A representative lot detected Wilms tumor protein WT1 in lysates from mouse E15.5 embryonic kidney and human melanoma cell lines A375, SK-MEL-28, and WM-266-4 (Wagner, N., et al. (2008). Pflugers Arch. Eur. J. Physiol. 455(5):839-847).
Immunocytochemistry Analysis: A representative lot immunostained the nucleus of methanol-fixed human melanoma A375 cells by fluorescent immunocytochemistry (Wagner, N., et al. (2008). Pflugers Arch. Eur. J. Physiol. 455(5):839-847).
Immunofluorescence Analysis: A representative lot immunostained the PCNA-positive nuclei of proliferating cells in formalin-fixed, paraffin-embedded human melanoma tissue sections by fluorescent immunohistochemistry (Wagner, N., et al. (2008). Pflugers Arch. Eur. J. Physiol. 455(5):839-847).
Immunohistochemistry Analysis: A representative lot immunostained glomeruli in formalin-fixed, paraffin-embedded normal human kidney and Wilms' tumor sections (Wagner, N., et al. (2008). Pediatr. Nephrol. 23(9):1445-1453).
Immunohistochemistry Analysis: A representative lot detected vascular WT1 expression in 95% of 113 paraffin-embedded tumour tissues of various types. In most cases, nuclear WT1 staining of endothelial cells was seen (Wagner, N., et al. (2008). Oncogene. 27(26):3662-3672).
Immunohistochemistry Analysis: A representative lot immunostained the nucleus of perifollicular fibroblasts at the hair follicle in formalin-fixed, paraffin-embedded normal human skin sections. Most common melanocytic nevi do not express WT1, whereas Spitz nevi and dysplastic nevi show cytoplasmic WT1 staining. (Wagner, N., et al. (2008). Pflugers Arch. Eur. J. Physiol. 455(5):839-847).
Biological Information
Immunogen
Recombinantt human WT-1 N-terminal fragment (a.a. 1-173).
Epitope
Amino acids 1-173.
Clone
6F-H2
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Expected to react with all spliced isoforms other than isoforms 6 and 9 reported by UniProt (P19544). Reactivity toward isoforms 6 and 9 has not been determined.
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Wilms tumor protein in 10 µg of Jurkat cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size
100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Bestellnummer
GTIN
MAB4234-C
04055977331288
Documentation
Anti-Wilms' Tumor Antibody, NT clone 6F-H2, Ascites Free SDB
