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MABS2028-25UG Anti-Vitamin D receptor Antibody, clone 9A7

Anti-Vitamin D receptor, clone 9A7, Cat. No. MABS2028, is a rat monoclonal antibody that detects Vitamin D3 receptor and has been tested for use in , Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Anti-Vitamin D receptor Antibody, clone 9A7: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Signaling Gene Symbol: VDR, NR111 UniProt Number: O42392
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MABS2028-25UG
25 μg  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, Ch, Porcine, RIH(P), IP, WBRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS2028-25UG
      DescriptionAnti-Vitamin D receptor Antibody, clone 9A7
      Alternate Names
      • VDR
      • 1
      • 25-dihydroxyvitamin D3 receptor
      • Nuclear receptor subfamily 1 group I member 1
      Background InformationVitamin D3 receptor (UniProt: O42392; also known as VDR, 1,25-dihydroxyvitamin D3 receptor, Nuclear receptor subfamily 1 group I member 1) is encoded by the VDR (also known as NR1I1) gene (Gene ID: 395988) in chicken. VDR is a nuclear receptor for calcitriol, the active form of vitamin D3 that mediates the action of vitamin D on cells. VDR is expressed mainly in kidney and intestine and plays a central role in calcium homeostasis. In the absence of bound vitamin D3 it is present as a homodimer and it enters the nucleus upon vitamin D3 binding where it forms heterodimers with the retinoid X receptor/RXR. The VDR-RXR heterodimers bind to specific response elements on DNA and activate the transcription of vitamin D3-responsive target genes. It is recruited to promoters via its interaction with BAZ1B/WSTF, which mediates the interaction with acetylated histones that is considered to be an essential step for VDR-promoter association. Plays a central role in calcium homeostasis. VDR is composed of three domains: a modulating N-terminal domain, a DNA-binding domain, and a C-terminal ligand-binding domain. Two isoforms of VDR have been described that are produced by alternative initiation.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Vitamin D receptor, clone 9A7, Cat. No. MABS2028, is a rat monoclonal antibody that detects Vitamin D3 receptor and has been tested for use in , Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation Analysis: A representative lot detected Vitamin D receptor in Immunoprecipitation applications (Pike, J.W., et. al. (1982). Proc Natl Acad Sci USA. 79(24); 7719-23).

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected Vitamin D receptor in human skin and human breast cancer tissue sections.
      Biological Information
      ImmunogenPartially purified chicken intestinal vitamin D receptor.
      Clone9A7
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 9A7 detects Vitamin D3 receptor in multiple avian and mammalian species.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Chicken
      • Porcine
      • Rat
      Species Reactivity NoteHuman, Chicken, Porcine, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • VDR
      • NR111
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~51 kDa observed; 51.3 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MCF-7 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Vitamin D receptor in MCF-7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABS2028-25UG 04054839510236