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AB1586 Anti-Ubiquitin Antibody

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AB1586
100 µL  
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      Übersicht

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Eu, H, Rb, ShWB, IHCShSerumPolyclonal Antibody
      Description
      Catalogue NumberAB1586
      Replaces04-454
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Ubiquitin Antibody
      Product Information
      FormatSerum
      PresentationSheep serum. Lyophilized, no preservatives. Reconstitute with 100 mL of sterile distilled water. Centrifuge to remove any residue.
      Quality LevelMQ100
      Applications
      ApplicationDetect Ubiquitin using this Anti-Ubiquitin Antibody validated for use in WB, IH.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry: 1:2,000-1:5,000.

      Immunoblotting: 1:1,000-1:2,000 (depending on detection system used).

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenUbiquitin from bovine red blood cells coupled to chicken gamma globulin.
      HostSheep
      SpecificityRecognizes both free ubiquitin and ubiquitin-protein conjugates on Western blots. Labels tangles and Lewy bodies in paraffin or frozen human brain sections.
      Species Reactivity
      • Eukaryote
      • Human
      • Rabbit
      • Sheep
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation. Ubiquitin also binds to histone H2A in actively transcribed regions but does not cause histone H2A degradation, suggesting that ubiquitin is also involved in regulation of gene expression. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. Aberrant form of this protein has been noticed in patients with Alzheimer's and Down syndrome.
      Gene Symbol
      • UBC
      • UBA80
      • HMG20
      • UBCEP1
      • Ubiquitin.
      • UBA52
      • UBB
      • UBCEP2
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P62988 # Protein modifier which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Attachment to proteins as a Lys-48-linked polymer usually leads to their degradation by proteasome. Attachment to proteins as a monomer or as an alternatively linked polymer does not lead to proteasomal degradation and may be required for numerous fonctions, including maintenance of chromatin structure, regulation of gene expression, stress response, ribosome biogenesis and DNA repair.
      SIZE: 76 amino acids; 8565 Da
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
      PTM: Several types of polymeric chains can be formed, depending on the lysine used for the assembly.
      SIMILARITY: SwissProt: P62988 ## Belongs to the ubiquitin family.
      MISCELLANEOUS: Ubiquitin is synthesized as a polyubiquitin precursor with exact head to tail repeats, the number of repeats differ between species and strains. In some species there is a final amino-acid after the last repeat, here in human a Val. Some ubiquitin genes contain a single copy of ubiquitin fused to a ribosomal protein (either L40 or S27a).
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20 to -70°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      Bestellnummer GTIN
      AB1586 04053252410024