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48-602MAG
Buffer Detection Kit for Magnetic Beads
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07-1431
Sigma-AldrichAnti-Tie-2 Antibody, NT
This Anti-Tie-2 Antibody, N-terminus is validated for use in WB for the detection of Tie-2.
More>>This Anti-Tie-2 Antibody, N-terminus is validated for use in WB for the detection of Tie-2. Less<<
Anti-Tie-2 Antibody, NT: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
venous malformations, multiple cutaneous and mucosal
Background Information
Tie-2 is a member of the tyrosine protein kinase family, is a transmembrane receptor for angiopoietin 1, and may represent one of the earliest markers for cell lineage within mammalian endothelial cells. Its expression is almost only found in human, mouse and rat endothelial cells and angioblasts. Most likely, Tie-2 regulates the differentiation, proper dispersal, and proliferation of these cells during angiogenesis. Mutations in TEK are a cause of VMCM or dominantly inherited venous malformations which are characterized by serpiginious, dilated veins. Tie-2 can be found in placenta and lung tissue. Lower levels of the protein can also be found in kidney, umbilical vein endothelial cells, and brain.
References
Product Information
Format
Affinity Purified
Control
Human brain lysate
Presentation
Affinity purified immunoglobulin. Liquid in 100 mM Tris, pH 7.5, containing 0.2% BSA and 0.05% sodium azide.
This Anti-Tie-2 Antibody, N-terminus is validated for use in WB for the detection of Tie-2.
Key Applications
Western Blotting
Biological Information
Immunogen
18 amino acid peptide sequence within the extracellular, N-terminus of human Tie-2
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
Recognizes human Tunica internal endothelial cell kinase (TIE-2), also described as Angiopoietin-1 receptor, p140 TEK, or tyrosine protein kinase receptor. The peptide sequence used as immunogen is 100% conserved in human, rat, mouse, and 94% in bovine.
FUNCTION: This protein is a protein tyrosine-kinase transmembrane receptor for angiopoietin 1. It may constitute the earliest mammalian endothelial cell lineage marker. Probably regulates endothelial cell proliferation, differentiation and guides the proper patterning of endothelial cells during blood vessel formation. CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. SUBUNIT STRUCTURE: Interacts with PTPRB. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Predominantly expressed in endothelial cells and their progenitors, the angioblasts. Has been directly found in placenta and lung, with a lower level in umbilical vein endothelial cells, brain and kidney. PTM: Phosphorylated. Phosphorylation is induced by ANGPT1 and ANGPT2. ANGPT1-induced phosphorylation is impaired during hypoxia. Dephosphorylated by PTPRB. INVOLVEMENT IN DISEASE: Defects in TEK are a cause of dominantly inherited venous malformations (VMCM) [MIM:600195]; an error of vascular morphogenesis characterized by dilated, serpiginous channels. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family. Tie subfamily. Contains 3 EGF-like domains. Contains 3 fibronectin type-III domains. Contains 2 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 protein kinase domain.
Molecular Weight
Approx. 126 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in human brain lysates. Western Blot Analysis: 1:1000 dilution of this lot detected Tie-2 in 10 µg of human brain lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.
Comparatine analysis of cytophenotypes of cells of mesenchymal lineage isolated from human tissues. Yu G Suzdal'tseva,V V Burunova,N V Petrakova,I V Vakhrushev,K N Yarygin,V N Yarygin Bulletin of experimental biology and medicine
143
2007
The expression of cytoplasmic and surface proteins in cultured human skin fibroblasts, human umbilical cord cells obtained after normal delivery on gestation week 38-40, and mesenchymal bone marrow stem cells was compared by the methods of immunocytochemistry and flow cytofluorometry. Bone marrow mesenchymal stem cells expressed a great variety of marker proteins typical of stem and progenitor cells and did not express proteins typical of differentiated cells. Fibroblast-like umbilical cord cells expressed markers of both stem cells and differentiated cells. Fibroblasts of dermal origin were characterized by intensive expression of proteins typical of differentiated cells.
The thin layer of cells that lines the interior surface of blood vessels and lymphatic vessels, forming an interface between circulating blood or lymph in the lumen and the rest of the vessel wall. Weitere Informationen >>
