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Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
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96-Well Plate
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48-602MAG
Buffer Detection Kit for Magnetic Beads
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ABE567
Sigma-AldrichAnti-THAP11 Antibody
Anti-THAP11 Antibody is a highly specific rabbit polyclonal antibody, that targets Ronin & has been tested in western blotting, IP & IHC.
More>>Anti-THAP11 Antibody is a highly specific rabbit polyclonal antibody, that targets Ronin & has been tested in western blotting, IP & IHC. Less<<
Anti-THAP11 Antibody: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
THAP11 (Ronin) is a transcriptional repressor which is predominantly expressed in embryonic stem (ES) cells. It contains a THAP-type zinc finger domain which is characteristic of the THAP 11 family members. In ES cells, THAP11 interacts with the transcriptional co-regulator HCF-1, to recruit chromatin-modifying proteins such as members of the polycomb family of proteins, to induce gene repression. THAP11 targets genes involved in metabolism, transcriptional initiation, and messenger RNA splicing. It plays an important role in maintaining the pluripotent state of ES cells.
References
Product Information
Format
Serum
Presentation
Flow through from affinity depleted rabbit polyclonal serum containing 0.05% sodium azide.
Anti-THAP11 Antibody is a highly specific rabbit polyclonal antibody, that targets Ronin & has been tested in western blotting, IP & IHC.
Key Applications
Western Blotting
Immunoprecipitation
Immunohistochemistry
Chromatin Immunoprecipitation (ChIP)
Application Notes
Western Blotting Analysis: A representative lot of an affinity purfied version of this antibody detected THAP11 in tested cell lysates (Parker, J. B., et al. (2012). Mol Cell Biol. 32(9):1654-1670.).
Immunoprecipitation Analysis: A representative lot of an an affinity purfied version of this antibody immunoprecipitated THAP11 in tested cell lysates (Parker, J. B., et al. (2012). Mol Cell Biol. 32(9):1654-1670.).
Chromatin Immunoprecipitation Analysis: A representative lot of an an affinity purfied version of this antibody immunoprecipitated THAP11 in SW620 cell lysates (Parker, J. B., et al. (2012). Mol Cell Biol. 32(9):1654-1670.).
Immunohistochemistry Analysis: A representative lot of an an affinity purfied version of this antibody detected THAP11 in normal colon epithelium and colon adenocarcinoma tissues, and in lymph node metastasis tissues (Parker, J. B., et al. (2012). Mol Cell Biol. 32(9):1654-1670.).
Biological Information
Immunogen
Recombinant protein corresponding to human THAP11.
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
~45 kDa observed. Uniprot describes a molecular weight at 35 kDa. However, this protein may be observed at ~45 kDa upon forming a complex with HCF-1. (Parker, J. B., et al. (2012). Mol Cell Biol 32(9):1654-1670.; Spanu, P. (1997). Gene. 193(1):89-96.).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HUVEC cell lysate.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected THAP11 in 10 µg of HUVEC cell lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
A transcriptional regulatory role of the THAP11-HCF-1 complex in colon cancer cell function. Parker, J Brandon, et al. Mol. Cell. Biol., 32: 1654-70 (2012)
2011
The recently identified Thanatos-associated protein (THAP) domain is an atypical zinc finger motif with sequence-specific DNA-binding activity. Emerging data suggest that THAP proteins may function in chromatin-dependent processes, including transcriptional regulation, but the roles of most THAP proteins in normal and aberrant cellular processes remain largely unknown. In this work, we identify THAP11 as a transcriptional regulator differentially expressed in human colon cancer. Immunohistochemical analysis of human colon cancers revealed increased THAP11 expression in both primary tumors and metastases. Knockdown of THAP11 in SW620 colon cancer cells resulted in a significant decrease in cell proliferation, and profiling of gene expression in these cells identified a novel gene set composed of 80 differentially expressed genes, 70% of which were derepressed by THAP11 knockdown. THAP11 was found to associate physically with the transcriptional coregulator HCF-1 (host cell factor 1) and recruit HCF-1 to target promoters. Importantly, THAP11-mediated gene regulation and its chromatin association require HCF-1, while HCF-1 recruitment at these genes requires THAP11. Collectively, these data provide the first characterization of THAP11-dependent gene expression in human colon cancer cells and suggest that the THAP11-HCF-1 complex may be an important transcriptional and cell growth regulator in human colon cancer.
