Wenn Sie das Fenster schließen, wird Ihre Konfiguration nicht gespeichert, es sei denn, Sie haben Ihren Artikel in die Bestellung aufgenommen oder zu Ihren Favoriten hinzugefügt.
Klicken Sie auf OK, um das MILLIPLEX® MAP-Tool zu schließen oder auf Abbrechen, um zu Ihrer Auswahl zurückzukehren.
Wählen Sie konfigurierbare Panels & Premixed-Kits - ODER - Kits für die zelluläre Signaltransduktion & MAPmates™
Konfigurieren Sie Ihre MILLIPLEX® MAP-Kits und lassen sich den Preis anzeigen.
Konfigurierbare Panels & Premixed-Kits
Unser breites Angebot enthält Multiplex-Panels, für die Sie die Analyten auswählen können, die am besten für Ihre Anwendung geeignet sind. Unter einem separaten Register können Sie das Premixed-Cytokin-Format oder ein Singleplex-Kit wählen.
Kits für die zelluläre Signaltransduktion & MAPmates™
Wählen Sie gebrauchsfertige Kits zur Erforschung gesamter Signalwege oder Prozesse. Oder konfigurieren Sie Ihre eigenen Kits mit Singleplex MAPmates™.
Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
.
Bestellnummer
Bestellinformationen
St./Pkg.
Liste
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Wählen Sie bitte Spezies, Panelart, Kit oder Probenart
Um Ihr MILLIPLEX® MAP-Kit zu konfigurieren, wählen Sie zunächst eine Spezies, eine Panelart und/oder ein Kit.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Catalogue Number
Ordering Description
Qty/Pack
List
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Spezies
Panelart
Gewähltes Kit
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
96-Well Plate
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
Weitere Reagenzien hinzufügen (MAPmates erfordern die Verwendung eines Puffer- und Detektionskits)
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Platzsparende Option Kunden, die mehrere Kits kaufen, können ihre Multiplex-Assaykomponenten in Kunststoffbeuteln anstelle von Packungen erhalten, um eine kompaktere Lagerung zu ermöglichen.
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Das Produkt wurde in Ihre Bestellung aufgenommen
Sie können nun ein weiteres Kit konfigurieren, ein Premixed-Kit wählen, zur Kasse gehen oder das Bestell-Tool schließen.
Anti-SCARB1, clone AOB-1, Cat. No. MABF3047, is a rat monoclonal antibody that detects SCARB1 and is tested for use in Flow Cytometry, Immunohistochemistry, Inhibition Assay, and Western Blotting.
More>>Anti-SCARB1, clone AOB-1, Cat. No. MABF3047, is a rat monoclonal antibody that detects SCARB1 and is tested for use in Flow Cytometry, Immunohistochemistry, Inhibition Assay, and Western Blotting. Less<<
Scavenger receptor class B member 1 (UniProt: Q61009; also known as SRB1, SR-BI) is encoded by the Scarb1 (also known as Srb1) gene (Gene ID: 20778) in murine species. SR-BI (SCARB1) is a widely expressed, multi-pass membrane glycoprotein with two cytoplasmic domains (aa 1-11 and 462-509), one extracellular domain (aa 33-440) and two transmembrane domains (aa 12-32 and 441-461). It serves as a receptor for several different ligands such as phospholipids, cholesterol ester, lipoproteins, and phosphatidylserine. It is a key component in the reverse cholesterol transport pathway and thus plays an important role in lipid metabolism. It may also contribute to variations in plasma lipid levels during fasting. Two isoforms of SR-BI have been described that are produced by alternative splicing and both isoforms can serve as a receptor for HDL and mediate selective uptake of cholesteryl ether and HDL-dependent cholesterol efflux. It is reported to facilitate the flux of free and esterified cholesterol between the cell surface and ApoB-containing lipoproteins and modified lipoproteins, although less efficiently than HDL. SR-BI is also involved in phagocytosis of apoptotic cells, via its phosphatidylserine binding activity. Elevated expression of SR-BI has been reported in a wide variety of malignant cell lines such as breast, prostate, ovarian, pancreatic, nasopharyngeal, and colorectal cancers. In hepatocytes, it is reported to act as a receptor for hepatitis C virus and facilitates virus entry into cells. SR-B1 is also described as a silica receptor that through an extracellular -helix specifically recognizes amorphous and crystalline silica. However, it does not recognize titanium dioxide nanoparticles, latex nanoparticles, or monosodium urate crystals. It is involved in silica-induced pulmonary inflammation in murine models. Clone AOB-1 is shown to specifically bind to murine SR-BI on Jurkat.EcoR cells and blocks silica binding to these cells in a dose-dependent manner. (Ref.: Tsugita, M., et al. (2017). Cell Rep. 18; 1298-1311; Mooberry, LK., et al. (2016). Front. Pharmacol. 7; 466). Webb, NR., et al. (1998). J. Biol. Chem. 273(24); 15241-15248).
References
Product Information
Format
Purified
Presentation
Purified rat monoclonal antibody IgG1 in PBS without azide.
Anti-SCARB1, clone AOB-1, Cat. No. MABF3047, is a rat monoclonal antibody that detects SCARB1 and is tested for use in Flow Cytometry, Immunohistochemistry, Inhibition Assay, and Western Blotting.
Key Applications
Flow Cytometry
Western Blotting
Immunohistochemistry
Inhibition
Application Notes
Tested Applications
Inhibition Assay; A representative lot of this antibody partially inhibited IL-1β secretion in silica stimulated bone marrow-derived macrophages (Tsugita, M., et al. (2017). Cell Rep.18(5); 1298-1311).
Flow Cytometry Analysis: A representative lot detected SCARB1 in Flow Cytometry application (Tsugita, M., et al. (2017). Cell Rep. 18(5);1298-1311).
Western Blotting Analysis: A representative lot detected SCARB1 in Western Blotting application (Tsugita, M., et al. (2017). Cell Rep. 18(5); 1298-1311).
Immunohistochemistry Applications: A representative lot detected SCARB1 in Immunohistochemistry applications (Tsugita, M., et al. (2017). Cell Rep.18(5);1298-1311).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
Immunogen
NIH3T3 cells transfected with mouse SCARB1 (SR-B1).
Epitope
Unknown
Clone
AOB-1
Concentration
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Host
Rat
Specificity
Clone AOB-1 is a rat monoclonal antibody that detects Scavenger receptor class B member 1 (SCARB1).
Evaluated by Flow Cytometry in mSR-B1/Jurkat.EcoR cells.
Flow Cytometry Analysis (FC): 1.0 µg of this antibody detected SR-B1 in one million Jurkat.EcoR cells expressing murine SR-B1 (SCRAB1).
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
