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553571 Anti-Ras (31-43) Rabbit pAb

Übersicht

Key Spec Table

Species ReactivityHostAntibody Type
B, H, M, RRbPolyclonal Antibody

Preis & Verfügbarkeit

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553571-50UG
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      Kst.-Ampulle 50 μg
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      Description
      OverviewRecognizes all forms of wild type Ras, including the mutant G12V Ras protein.
      Catalogue Number553571
      Brand Family Calbiochem®
      SynonymsAnti-Ras GDP/GTP Binding Protein
      Application Data
      Detection of rat Ras by immunoblotting. Sample: Whole tissue extract from rat brain. Primary antibody: Anti-Ras (31-43) Rabbit pAb (Cat. No. 553571) (1 µg/ml). Detection: chemiluminescence.
      References
      ReferencesHardie, G. and Hanks, S. 1995. in The Protein Kinase Facts Book, Protein-Serine Kinases p. 418, Academic Press, San Diego, CA.
      Sawada, M., et al. 1989. Nucleic Acid Res. 17, 8867.
      Product Information
      FormLiquid
      FormulationIn PBS, 50% glycerol, pH 7.2.
      Positive controlRat brain tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1 µg/ml)
      Application CommentsBy immmunoblotting, this anti-pan-Ras polyclonal antibody reacts strongly with recombinant Ras fusion protein as well as with recombinant glutathione S-transferase (GST) human Ras and [G12V]GST human Ras. Does not cross-react with members of the Rho or Rab families of Ras-related small G-proteins. No specific reactivity is detected by immunoblotting with rat, mouse or human cell lines or tissue extracts. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic peptide [EYDPTIEDSYRKQ(C)] corresponding to amino acids 31-43 in the effector binding Loop (L2) of human H-, K-, and N-Ras, conjugated to KLH
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Bovine
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Global Trade Item Number
      Bestellnummer GTIN
      553571-50UG 04055977193756