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MABS1234 Anti-Progesterone Receptor B Antibody, clone 250/H11

This Anti-Progesterone Receptor B Antibody, clone 250/H11 is validated for use in Western Blotting and Immunocytochemistry for the detection of Progesterone Receptor B.

SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: PGR, NR3C3 UniProt Number: P06401
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MABS1234
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1234
      DescriptionAnti-Progesterone Receptor B Antibody, clone 250/H11
      Alternate Names
      • Progesterone receptor
      • PR
      • Nuclear receptor subfamily 3 group C member 3
      • Progesterone Receptor B
      Background InformationProgesterone receptor (UniProt P06401; also known as Nuclear receptor subfamily 3 group C member 3, PR) is encoded by the PGR (also known as NR3C3) gene (Gene ID 5241) in human. The progesterone receptor (PR) is a hormone activated transcription factor, where hormone binding triggers the dissociation of heat shock and chaperone proteins from PR, leading to PR dimerization and activation. The receptor dimer then binds progestin response elements in the regulatory regions of its target genes and a multi-component complex is assembled to enable transcription. In addition, a number of kinases are also reported to regulate PR activity via phosphorylation. PR can also regulate transcription without direct DNA binding by tethering to other transcription factors, such as specificity protein 1 (Sp1) or activator protein 1 (AP-1), and modulating their transcriptional activity on target genes. PR is expressed as five isoforms, including PR-B and PR-A that are produced as a result of alternate estrogen inducible promoters within the same PGR gene. PR-A lacks the first 164 amino acids of PR-B but the two isoforms have otherwise identical sequence. PR-B and PR-A can regulate distinct subsets of target genes and studies in knockout mice suggest that PR-B predominantly regulates mammary gland development and PR-A is critical for normal uterine function.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Progesterone Receptor B Antibody, clone 250/H11 is validated for use in Western Blotting and Immunocytochemistry for the detection of Progesterone Receptor B.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A representative lot stained formalin-fixed and paraffin-embedded MCF10A cells engineered to express progesterone receptor isoform B (PR-B), but not MCF10A cells engineered to express isoform A/PR-A (Courtesy of Dr. Dean Edwards, Baylor College of Medicine, USA).
      Immunocytochemistry Analysis: A representative lot stained paraformaldehyde-fixed and Triton X-100-fixed MCF10A cells engineered to express progesterone receptor isoform B (PR-B), but not MCF10A cells engineered to express isoform A/PR-A by fluorescent immunocytochemistry (Courtesy of Dr. Dean Edwards, Baylor College of Medicine, USA).
      Western Blotting Analysis: A representative lot detected endogenous progesterone receptor isoform B (PR-B) in human breast cancer T47D cell lysate, as well as exogenously expressed PR-B in transfected DCIS.COM human ductal carcinoma in situ (DCIS) breast cancer cells, but not in PR-A-transfected or untransfected DCIS.COM cells (Courtesy of Dr. Dean Edwards, Baylor College of Medicine, USA).
      Biological Information
      ImmunogenRecombinant protein corresponding to the N-terminal of human Progesterone Receptor B.
      EpitopeN-terminal
      Clone250/H11
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 250/H11 reacts with isoform 1 (isoform B; PR-B), but not isoform 2 (isoform A; PR-A). This clone is also expected to react with isoform 5 (delta4), but not isoform 3 or 4 (PR-M).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PGR
      • NR3C3
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~110 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human breast cancer T47D cell lysate.

      Western Blotting Analysis: 2.0 µg/mL of this antibody detected progesterone receptor isoforms B (PR-B), but not isoform A (PR-A) in human breast cancer T47D cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABS1234 04055977288391

      Documentation

      Anti-Progesterone Receptor B Antibody, clone 250/H11 SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Anti-Progesterone Receptor B Antibody, clone 250/H11 Analysenzertifikate

      TitelChargennummer
      Anti-Progesterone Receptor B, -Q2606953 Q2606953
      Anti-Progesterone Receptor B, clone 250/H11 - 3245597 3245597
      Anti-Progesterone Receptor B, clone 250/H11 - 3682534 3682534

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      Kategorien

      Life Science Research > Antibodies and Assays > Primary Antibodies