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16-101 Anti-Phosphotyrosine Antibody, clone 4G10®, agarose conjugate

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16-101
1 mg  
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      Übersicht

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      VrtIPMGel ImmobilizedMonoclonal Antibody
      Description
      Catalogue Number16-101
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Phosphotyrosine Antibody, clone 4G10®, agarose conjugate
      OverviewAnti-Phosphotyrosine antibody (05-321), cross-linked to protein A agarose by dimethylpimelimidate
      Background InformationSome of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity. The advent of anti-phospho-tyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
      Product Information
      FormatGel Immobilized
      Control
      • Pervanadate-treated human A431 cell extracts, EGF-treated human A431 cells.
      Presentation1 mg of Anti-Phosphotyrosine monoclonal antibody covalently coupled to 1 mL of protein A agarose beads and provided as a 50% slurry suspended in PBS, containing 0.05% Kathon ® as a preservative, for a total volume of 2 mL. Liquid suspension.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Phosphotyrosine Antibody, clone 4G10®, agarose conjugate is an antibody against Phosphotyrosine for use in IP.
      Key Applications
      • Immunoprecipitation
      Application NotesThis product is useful for immunoprecipitation and immunoaffinity chromatography methods.

      Suitable for immunoprecipitation and affinity purification.
      Biological Information
      ImmunogenPhosphotyramine-KLH.
      Clone4G10®,
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes tyrosine-phosphorylated proteins from all species.
      Species Reactivity
      • Vertebrates
      Antibody TypeMonoclonal Antibody
      Modifications
      • Phosphorylation
      Purification MethodProtein A Purfied
      Molecular WeightDependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
      Product Usage Statements
      Quality AssuranceEvaluated by immunoprecipitating the EGF receptor and other tyrosine phosphorylated proteins from EGF-stimulated A431 cells.

      Immunoprecipitation Analysis:
      10-50 μL of this antibody immunoprecipitated the EGF receptor and other tyrosine phosphorylated proteins from EGFstimulated A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size1 mg
      Global Trade Item Number
      Bestellnummer GTIN
      16-101 04053252514951