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ST1517 Anti-PIP5K3 Mouse mAb (6C7)

This Anti-PIP5K3 Mouse mAb (6C7) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of PIP5K3.

Anti-PIP5K3 Mouse mAb (6C7): SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

Synonyme: Anti-Phosphatidylinosi-3-Phosphate-Phosphatidylinosi-5-Kinase Type 3

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ST1517
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Übersicht

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

Preis & Verfügbarkeit

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ST1517-100UG
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      		 100 μg
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      Description
      OverviewRecognizes the ~60 kDa PIP5K3 protein in human testes tissue and HeLa cells.
      Catalogue NumberST1517
      Brand Family Calbiochem®
      SynonymsAnti-Phosphatidylinosi-3-Phosphate-Phosphatidylinosi-5-Kinase Type 3
      Application Data
      Detection of human PIP5K3 by immunoblotting. Sample: Whole cell lysate from 293T cells transfected with full-length human PIP5K3 (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-PIP5K3 Mouse mAb (6C7) (Cat. No. ST1517) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human PIP5K3 by immunoblotting. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-PIP5K3 Mouse mAb (6C7) (Cat. No. ST1517) (10 µg/ml). Detection: fluorescence.

      Detection of human PIP5K3 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human testes tissue. Primary antibody: Anti-PIP5K3 Mouse mAb (6C7) (Cat. No. ST1517) (1-5 µg/ml). Detection: colorimetric (DAB).
      References
      ReferencesTsuruta, F., et al. 2009. J. Cell Biol. 187 279.
      Product Information
      Formliquid
      FormulationIn PBS, pH 7.2.
      Negative control293T cells
      Positive controlHuman testes tissue, HeLa cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Paraffin Sections
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids 342-452 of human PIP5K3, expressed as a GST fusion protein
      ImmunogenHuman
      Clone6C7
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ST1517-100UG 04055977224184

      Documentation

      Anti-PIP5K3 Mouse mAb (6C7) SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Literatur

      Übersicht
      Tsuruta, F., et al. 2009. J. Cell Biol. 187 279.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-November-2010 RFH
      SynonymsAnti-Phosphatidylinosi-3-Phosphate-Phosphatidylinosi-5-Kinase Type 3
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application Data
      Detection of human PIP5K3 by immunoblotting. Sample: Whole cell lysate from 293T cells transfected with full-length human PIP5K3 (lane 1) and from non-transfected 293T cells (lane 2). Primary antibody: Anti-PIP5K3 Mouse mAb (6C7) (Cat. No. ST1517) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human PIP5K3 by immunoblotting. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-PIP5K3 Mouse mAb (6C7) (Cat. No. ST1517) (10 µg/ml). Detection: fluorescence.

      Detection of human PIP5K3 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human testes tissue. Primary antibody: Anti-PIP5K3 Mouse mAb (6C7) (Cat. No. ST1517) (1-5 µg/ml). Detection: colorimetric (DAB).
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~60 kDa PIP5K3 protein.
      BackgroundPIP5K3 belongs to a large family of lipid kinases that alter the phosphorylation status of intracellular phosphatidylinositol. Signaling by phosphorylated species of phosphatidylinositol regulates diverse cellular processes, including membrane trafficking and cytoskeletal reorganization.
      HostMouse
      Immunogen speciesHuman
      ImmunogenA recombinant polypeptide corresponding to amino acids 342-452 of human PIP5K3, expressed as a GST fusion protein
      Clone6C7
      IsotypeIgG2a
      Specieshuman
      Positive controlHuman testes tissue, HeLa cells
      Negative control293T cells
      Formliquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review
      ReferencesTsuruta, F., et al. 2009. J. Cell Biol. 187 279.