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AB5407 Anti-Opsin Antibody, blue

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AB5407
100 µg  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, MkIH(P)RbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB5407
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Opsin Antibody, blue
      Background InformationThe full range of color discrimination in humans is based on the presence and function of three cone photoreceptor mechanisms. Each cone type possesses a photo-sensitive pigment-protein complex consisting of 11-cis retinal and a unique opsin protein, which gives sensitivity in the short (S cone, peak sensitivity about 420nm), middle (M cone, peak sensitivity about 530nm with polymorphism; Winderckx et al., 1993; Neitz & Neitz, 1998), and long (L cone, peak sensitivity about 560nm with polymorphism; Neitz & Jacobs, 1990) wavelengths of the light spectrum. All three opsins are transmembrane proteins with seven membrane-spanning regions. Genes for the three types of cone opsins and the rod photoreceptor rhodopsin gene seem to be homologous with varying amounts of conservation. Strongest conservation is between the middle (green) and long (red) wavelength sensitive pigments on the X chromosome, suggesting a relatively recent duplication/divergence event (Nathans, 1989; Nathans et al., 1992). The S cone (blue) opsin is located on chromosome 7 and seems to have stronger conservation with rhodopsin. Cone photoreceptor distribution in humans is dominated by the M and L cone pigments.
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      Control
      • Retina
      PresentationPurified immunoglobulinin PBS {0.02M phosphate, 0.25M NaCl, pH 7.6} with 0.1% sodium azide as a preservative
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Opsin Antibody, blue is validated for use in IH(P) for the detection of Opsin.
      Key Applications
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry: 1:200-1:300 on formalin-fixed, paraffin-embedded mouse retina tissue. Antigen retrieval method recommend is HIER with steam heat; other fixation and retrieval methods are untested.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenRecombinant human blue opsin.
      Epitopeblue
      HostRabbit
      SpecificityRecognizes Opsin, blue.
      Species Reactivity
      • Human
      • Mouse
      • Monkey
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • OPN1SW
      • CBT
      • BOP
      • BCP
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P03999 # Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal.
      SIZE: 348 amino acids; 39135 Da
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: The three color pigments are found in the cone photoreceptor cells.
      PTM: Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
      DISEASE:SwissProt: P03999 # Defects in OPN1SW are the cause of tritan color blindness (tritanopia) [MIM:190900].SIMILARITY:SwissProt: P03999 ## Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain for 1 year at 2–8°C from date of shipment.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      AB5407 04053252468544

      Documentation

      Anti-Opsin Antibody, blue SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Anti-Opsin Antibody, blue Analysenzertifikate

      TitelChargennummer
      RABBIT ANTI-OPSIN, BLUE POLYCLONAL ANTIBODY - 2424750 2424750
      RABBIT ANTI-OPSIN, BLUE POLYCLONAL ANTIBODY - 2462859 2462859
      RABBIT ANTI-OPSIN, BLUE POLYCLONAL ANTIBODY - 2011455 2011455
      RABBIT ANTI-OPSIN, BLUE - 3224643 3224643
      RABBIT ANTI-OPSIN, BLUE - 3383998 3383998
      RABBIT ANTI-OPSIN, BLUE - 3438565 3438565
      RABBIT ANTI-OPSIN, BLUE - 3609330 3609330
      RABBIT ANTI-OPSIN, BLUE - 3756539 3756539
      RABBIT ANTI-OPSIN, BLUE - 3851537 3851537
      RABBIT ANTI-OPSIN, BLUE - 3954349 3954349

      Literatur

      ÜbersichtAnwendungSpeziesPub Med ID
      AAV-mediated RLBP1 gene therapy improves the rate of dark adaptation in Rlbp1 knockout mice.
      Choi, VW; Bigelow, CE; McGee, TL; Gujar, AN; Li, H; Hanks, SM; Vrouvlianis, J; Maker, M; Leehy, B; Zhang, Y; Aranda, J; Bounoutas, G; Demirs, JT; Yang, J; Ornberg, R; Wang, Y; Martin, W; Stout, KR; Argentieri, G; Grosenstein, P; Diaz, D; Turner, O; Jaffee, BD; Police, SR; Dryja, TP
      Molecular therapy. Methods & clinical development  2  15022  2015

      Abstract anzeigen
      26199951 26199951
      Localization of complement factor H gene expression and protein distribution in the mouse outer retina.
      Smit-McBride, Z; Oltjen, SL; Radu, RA; Estep, J; Nguyen, AT; Gong, Q; Hjelmeland, LM
      Molecular vision  21  110-23  2015

      Abstract anzeigen
      25684976 25684976
      CRALBP supports the mammalian retinal visual cycle and cone vision.
      Xue, Y; Shen, SQ; Jui, J; Rupp, AC; Byrne, LC; Hattar, S; Flannery, JG; Corbo, JC; Kefalov, VJ
      The Journal of clinical investigation  125  727-38  2015

      Abstract anzeigen
      25607845 25607845
      Mutations in the unfolded protein response regulator ATF6 cause the cone dysfunction disorder achromatopsia.
      Kohl, S; Zobor, D; Chiang, WC; Weisschuh, N; Staller, J; Gonzalez Menendez, I; Chang, S; Beck, SC; Garcia Garrido, M; Sothilingam, V; Seeliger, MW; Stanzial, F; Benedicenti, F; Inzana, F; Héon, E; Vincent, A; Beis, J; Strom, TM; Rudolph, G; Roosing, S; Hollander, AI; Cremers, FP; Lopez, I; Ren, H; Moore, AT; Webster, AR; Michaelides, M; Koenekoop, RK; Zrenner, E; Kaufman, RJ; Tsang, SH; Wissinger, B; Lin, JH
      Nature genetics  47  757-65  2015

      Abstract anzeigen
      Western Blotting26029869 26029869
      Gene Therapy Fully Restores Vision to the All-Cone Nrl(-/-) Gucy2e(-/-) Mouse Model of Leber Congenital Amaurosis-1.
      Boye, SL; Peterson, JJ; Choudhury, S; Min, SH; Ruan, Q; McCullough, KT; Zhang, Z; Olshevskaya, EV; Peshenko, IV; Hauswirth, WW; Ding, XQ; Dizhoor, AM; Boye, SE
      Human gene therapy  26  575-92  2015

      Abstract anzeigen
      26247368 26247368
      Long-term retinal cone survival and delayed alteration of the cone mosaic in a transgenic mouse model of stargardt-like dystrophy (STGD3).
      Kuny, S; Filion, MA; Suh, M; Gaillard, F; Sauvé, Y
      Investigative ophthalmology & visual science  55  424-39  2014

      Abstract anzeigen
      24334447 24334447
      Three distinct blue-green color pathways in a mammalian retina.
      Mills, SL; Tian, LM; Hoshi, H; Whitaker, CM; Massey, SC
      The Journal of neuroscience : the official journal of the Society for Neuroscience  34  1760-8  2014

      Abstract anzeigen
      24478358 24478358
      Natural history of cone disease in the murine model of Leber congenital amaurosis due to CEP290 mutation: determining the timing and expectation of therapy.
      Boye, SE; Huang, WC; Roman, AJ; Sumaroka, A; Boye, SL; Ryals, RC; Olivares, MB; Ruan, Q; Tucker, BA; Stone, EM; Swaroop, A; Cideciyan, AV; Hauswirth, WW; Jacobson, SG
      PloS one  9  e92928  2014

      Abstract anzeigen
      24671090 24671090
      Onecut1 and Onecut2 redundantly regulate early retinal cell fates during development.
      Sapkota, D; Chintala, H; Wu, F; Fliesler, SJ; Hu, Z; Mu, X
      Proceedings of the National Academy of Sciences of the United States of America  111  E4086-95  2014

      Abstract anzeigen
      25228773 25228773
      Independent genomic control of neuronal number across retinal cell types.
      Keeley, PW; Whitney, IE; Madsen, NR; St John, AJ; Borhanian, S; Leong, SA; Williams, RW; Reese, BE
      Developmental cell  30  103-9  2014

      Abstract anzeigen
      24954025 24954025