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Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
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Anti-Nuf2 Antibody, clone 28-37 is a high quality Mouse Monoclonal Antibody for the detection of Nuf2 & has been validated in IP, WB, ICC.
More>>Anti-Nuf2 Antibody, clone 28-37 is a high quality Mouse Monoclonal Antibody for the detection of Nuf2 & has been validated in IP, WB, ICC. Less<<
Anti-Nuf2 Antibody, clone 28-37: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Nuf2 is a protein part of a complex of kinetochore proteins that serve to attach microtubules to the kinetochore. Also called CDCA1. Members of the Ndc80/Nuf2 complex have been shown in several systems to be important in formation of stable kinetochore-microtubule attachments and chromosome alignment in mitosis. In HeLa cells, it has been shown that depletion of Nuf2 by RNAi results in a strong prometaphase block with an active spindle checkpoint. A smaller 320 AA splice variant would not be recognized by this ab.
References
Product Information
Format
Purified
Applications
Application
Anti-Nuf2 Antibody, clone 28-37 is a high quality Mouse Monoclonal Antibody for the detection of Nuf2 & has been validated in IP, WB, ICC.
Key Applications
Immunoprecipitation
Western Blotting
Immunocytochemistry
Biological Information
Immunogen
His-tagged fusion protein corresponding to full length human Nuf2
This gene encodes a protein that is highly similar to yeast Nuf2, a component of a conserved protein complex associated with the centromere. Yeast Nuf2 disappears from the centromere during meiotic prophase when centromeres lose their connection to the spindle pole body, and plays a regulatory role in chromosome segregation. The encoded protein is found to be associated with centromeres of mitotic HeLa cells, which suggests that this protein is a functional homolog of yeast Nuf2. Alternatively spliced transcript variants that encode the same protein have been described.
FUNCTION: SwissProt: Q9BZD4 # Acts as a component of the essential kinetochore- associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. SIZE: 464 amino acids; 54304 Da SUBUNIT: Component of the NDC80 complex, which consists of KNTC2/HEC1, CDCA1, SPBC24 and SPBC25. The NDC80 complex is formed by two subcomplexes composed of KNTC2/HEC1-CDCA1 and SPBC24- SPBC25. Each subcomplex is formed by parallel interactions through the coiled-coil domains of individual subunits. Formation of a tetrameric complex is mediated by interactions between the C- terminal regions of both subunits of the KNTC2/HEC1-CDCA1 subcomplex and the N-terminal regions of both subunits of the SPBC24-SPBC25 complex. The tetrameric NDC80 complex has an elongated rod-like structure with globular domains at either end. May interact with AURKB/Aurora-B. SUBCELLULAR LOCATION: Nucleus. Kinetochore. Note=Localizes to kinetochores from late prophase to anaphase. Localizes specifically to the outer plate of the kinetochore. PTM: Can be phosphorylated by AURKA and AURKB. SIMILARITY: SwissProt: Q9BZD4 ## Belongs to the NUF2 family.
Molecular Weight
Mr 53kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
routinely evaluated by immunoblot on RIPA lysates from acid extracted HeLa cells
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Kinetochore localization and microtubule interaction of the human spindle checkpoint kinase Mps1. Stucke, Volker M, et al. Chromosoma, 113: 1-15 (2004)
2004
Members of the Mps1 protein kinase family have been implicated in the regulation of the kinetochore-mediated spindle assembly checkpoint in species ranging from yeast to man. However, conflicting data have been reported on the subcellular localization of vertebrate Mps1 kinases and their possible roles in centrosome duplication. Moreover, little is presently known about the regulation of Mps1 kinases during the cell cycle. Here, we have used immunofluorescence microscopy, immunoblotting and siRNA-mediated depletion of hMps1 to re-investigate the subcellular localization of this kinase. Our data confirm the kinetochore association of hMps1 but suggest that the centrosome staining produced by some anti-hMps1 antibodies could be due to cross-reactivity with other proteins. We also show that the kinetochore association of hMps1 is mediated by the amino-terminal, non-catalytic domain and specifically requires the presence of the Hec1/Ndc80-Nuf2 complex at the kinetochore. Finally, we have combined in vitro binding studies and kinase assays to explore the influence of microtubules on hMps1 activity. Our data indicate that the catalytic domain of hMps1 displays affinity for microtubules and that microtubule binding could contribute to the regulation of kinase activity.
