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PC223L Anti-Neuropeptide Y Rabbit pAb

This Anti-Neuropeptide Y Rabbit pAb is validated for use in Frozen Sections for the detection of Neuropeptide Y.

Anti-Neuropeptide Y Rabbit pAb: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
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PC223L
Preis & Verfügbarkeit

Übersicht

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
Am, Av, Fe, F, H, Newt, RRbPolyclonal Antibody

Preis & Verfügbarkeit

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PC223L-100UL
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      Description
      OverviewRecognizes neuropeptide Y in cortex, caudate, putamen, and paraventricular nucleus.
      Catalogue NumberPC223L
      Brand Family Calbiochem®
      Application Data
      Detection of rat neuropeptide Y by immunohistochemistry. Sample: Rat paraventricular nucleus, cortex, and caudate putamen. Primary antibody: Anti-Neuropeptide Y Rabbit pAb (Cat. No. PC223L) (1:10,000). Detection: fluorescence.
      References
      ReferencesSudhof, T.C. 1995. Nature 375, 645.
      Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.
      Product Information
      FormLyophilized
      FormulationUndiluted serum.
      Positive controlParaventricular nucleus, cortex, or caudate putamen
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Frozen Sections
      Application NotesFrozen Sections (1:1000-1:2000 for free-floating, PAP detection)
      Application CommentsAntibody specificity was examined in the rat cortex, caudate putamen, and paraventricular nucleus. Staining is completely eliminated by pretreatment of antibody with neuropeptide Y (10-6M). Preabsorption with avian pancreatic polypeptide, β-endorphin, cholecystokinin, peptide YY, somatostatin, or vasoactive intestinal peptide resulted in no loss of immunostaining. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenporcine neuropeptide Y
      ImmunogenPorcine
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Amphibian
      • Avian
      • Feline
      • Fish
      • Human
      • Newt
      • Rat
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      PC223L-100UL 04055977224672

      Documentation

      Anti-Neuropeptide Y Rabbit pAb SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Anti-Neuropeptide Y Rabbit pAb Analysenzertifikate

      TitelChargennummer
      PC223L

      Literatur

      Übersicht
      Sudhof, T.C. 1995. Nature 375, 645.
      Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.
      Datenblatt

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision09-October-2007 RFH
      ApplicationFrozen Sections (1:1000-1:2000 for free-floating, PAP detection)
      Application Data
      Detection of rat neuropeptide Y by immunohistochemistry. Sample: Rat paraventricular nucleus, cortex, and caudate putamen. Primary antibody: Anti-Neuropeptide Y Rabbit pAb (Cat. No. PC223L) (1:10,000). Detection: fluorescence.
      DescriptionRabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes neuropeptide Y.
      BackgroundNeurotransmitters can be defined as chemical substances that are released by the presynaptic neuron before being bound by receptors on the postsynaptic cell surface. It has been well established that the nervous system utilizes neurotransmitters for cell signaling. Neurotransmitters can be divided into two groups; low molecular weight transmitter molecules such as biogenic amines, purines, or amino acids, and bioactive peptides, of which more than 50 members have been identified in the mammalian central nervous system. Many low molecular weight neurotransmitters are produced within the axon terminal from common cellular metabolites and packaged into synaptic vesicles. In contrast, neuropeptides are synthesized in the cell body and packaged into large dense core vesicles for delivery to the presynaptic membrane. The transport and delivery of vesicles containing neurotransmitter is a highly regulated and complex process. Neuropeptide Y (NPY) is a 36 amino acid peptide belonging to the pancreatic polypeptide family of neuroendocrine hormones. NPY is abundantly expressed in neurons within the central and peripheral nervous systems, including the hypothalamus and pituitary. NPY is a potent stimulator of food intake, and may also influence other growth hormones. A family of neuropeptide Y receptors (Y1-Y5) have been identified which recognize both neuropeptide Y and the related Peptide YY. The immunolocalization, neuronal distribution, and vesicular compartmentalization of neurotransmitter molecules and their precursors are important for understanding the modulation, regulation, and function of neurotransmitters in the nervous system.
      HostRabbit
      Immunogen speciesPorcine
      Immunogenporcine neuropeptide Y
      IsotypeIgG
      Speciesamphibian, avian, feline, fish, human, newt, rat
      Positive controlParaventricular nucleus, cortex, or caudate putamen
      FormLyophilized
      FormulationUndiluted serum.
      Preservative≤0.1% sodium azide
      CommentsAntibody specificity was examined in the rat cortex, caudate putamen, and paraventricular nucleus. Staining is completely eliminated by pretreatment of antibody with neuropeptide Y (10-6M). Preabsorption with avian pancreatic polypeptide, β-endorphin, cholecystokinin, peptide YY, somatostatin, or vasoactive intestinal peptide resulted in no loss of immunostaining. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. Be careful to reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesSudhof, T.C. 1995. Nature 375, 645.
      Hokfelt, T., et al. 1987. Experientia Suppl. 56, 154.