Millipore Sigma Vibrant Logo

ABN90P Anti-NeuN purified Antibody

View Products on Sigmaaldrich.com
ABN90P
100 µL  
Preis wird abgerufen...
Preis nicht abrufbar
Die Mindestmenge muss ein Vielfaches sein von
Maximum Quantity is
Bei Bestätigung Weitere Informationen
Sie haben () gespart
 
Bitte erfragen
Eingeschränkte Verfügbarkeit
Eingeschränkte Verfügbarkeit
Lieferbar 
Produkt wurde eingestellt
Begrenzter Lagerbestand
Bestätigung der Verfügbarkeit erforderlich
    Restmenge: Angebot folgt
      Restmenge: Angebot folgt
      Bitte erfragen
      Kontakt zum Kundenservice
      Contact Customer Service

      Sonderaktionen

       

      Kontakt zum Kundenservice

      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWB, IH(P), ICCGpAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN90P
      DescriptionAnti-NeuN purified Antibody
      Alternate Names
      • NEUronal Nuclei
      • clone A60
      • NeuN
      Background InformationRNA binding protein fox-1 homolog 3 (UniProt: Q8BIF2; also known as Fox-1 homolog C, Hexaribonucleotide-binding protein 3, Fox-3, Neuronal nuclei antigen, NeuN antigen) is encoded by the Rbfox3 (also known as D11Bwg0517e, Hrnbp3) gene (Gene ID: 52897) in murine species. The NeuN is localized in nuclei and perinuclear cytoplasm of most of the neurons in the central nervous system of mammals. It is widely expressed in various regions of the brain, including the cerebral cortex, hippocampus, thalamus, caudate/putamen, cerebellum, as well as in the spinal cord. However, it is not detected in Cajal-Retzius cells in the neocortex, Purkinje cells, and photoreceptor cells. NeuN expression has been reported as early as embryonic day 9.5 (E9.5) and by E12.5 it can be found in the developing ventral horns and is also detected in the developing dorsal horns and in the dorsal root ganglion. It is expressed from embryonic stage to adulthood. Its expression is enhanced by retinoic acid treatment. It can serve as a pre-mRNA alternative splicing regulator and regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay. Anti-NeuN antibodies can identify most types of neurons in the whole nervous system with some rare exceptions. Antibodies for NeuN predominantly associate with cell nuclei and, to a lesser extent, with the perinuclear cytoplasm. Two isoforms of the NeuN protein (46 and 48 kDa) are present in both locations but differ in their relative concentration in the nucleus and cytoplasm.NeuN knockout mice display significantly reduced brain weight, impaired neurofilament expression and decreased white matter volume. They show increased susceptibility to seizures and reduced anxiety-related behaviors compared with wild type littermates. (Ref.: Lin, YS et al. (2018). PLOS One. 13(2); e0192355; Mao, S., et al. (2016). Front. Neuroanat. 13;10:54).
      References
      Product Information
      FormatAffinity Purified
      Control
      • Mouse E16 brain tissue lysate
      PresentationPurified guinea pig polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-NeuN purified, Cat. No. ABN90P, is a guinea pig polyclonal antibody that detects NeuN and is tested for use in Western Blotting and Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Immunocytochemistry
      Application NotesTested Applications

      Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected NeuN in mouse brain tissue sections.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to the first 97 amino acids of mouse NeuN.
      EpitopeN-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostGuinea Pig
      SpecificityThis Guinea pig polyclonal antibody detects NeuN. It targets an epitope within the N-terminal region.
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. Predicted to react with Human, Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • NeuN
      Purification MethodAntigen Affinity Purified
      Molecular Weight~48 kDa observed; 40.61 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in E16 Mouse brain tissue lysate.

      Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected NeuN in E16 Mouse brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ABN90P 04053252647635

      Documentation

      Anti-NeuN purified Antibody SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Anti-NeuN purified Antibody Analysenzertifikate

      TitelChargennummer
      Anti-NeuN purified 3083062
      Anti-NeuN purified - 2123666 2123666
      Anti-NeuN purified - 2445032 2445032
      Anti-NeuN purified - 2069374 2069374
      Anti-NeuN purified - 2145116 2145116
      Anti-NeuN purified - 2201953 2201953
      Anti-NeuN purified - 2272164 2272164
      Anti-NeuN purified - 2329178 2329178
      Anti-NeuN purified - 3158068 3158068
      Anti-NeuN purified - 3226535 3226535

      Literatur

      ÜbersichtPub Med ID
      Diffusion tensor and volumetric magnetic resonance measures as biomarkers of brain damage in a small animal model of HIV.
      Lentz, MR; Peterson, KL; Ibrahim, WG; Lee, DE; Sarlls, J; Lizak, MJ; Maric, D; Reid, WC; Hammoud, DA
      PloS one  9  e105752  2014

      Abstract anzeigen
      25144656 25144656

      Broschüre

      Titel
      New Products: Volume 3, 2012

      Verwandte Produkte & Anwendungen

      Related Products

      Bestellnummer Beschreibung  
      ABN78 Anti-NeuN Antibody (rabbit) Preis & Verfügbarkeit
      ABN78A4 Anti-NeuN (rabbit) Antibody, Alexa Fluor® 488 Conjugate Preis & Verfügbarkeit
      ABN78B Anti-NeuN (rabbit) Antibody, Biotin Conjugate Preis & Verfügbarkeit
      ABN78C3 Anti-NeuN (rabbit) Antibody, Cy3 Conjugate Preis & Verfügbarkeit
      ABN90 Anti-NeuN Antibody Preis & Verfügbarkeit
      AP108P Goat Anti-Guinea Pig IgG Antibody, HRP conjugate Preis & Verfügbarkeit
      MAB377 Anti-NeuN Antibody, clone A60 Preis & Verfügbarkeit

      Kategorien

      Life Science Research > Antibodies and Assays > Primary Antibodies