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ABN90
Sigma-AldrichAnti-NeuN Antibody
This guinea pig polyclonal Anti-NeuN, Cat. No. ABN90 is tested for use in Western Blotting, Immunocytochemistry and Immunohistochemistry (Paraffin), for the detection of NeuN.
More>>This guinea pig polyclonal Anti-NeuN, Cat. No. ABN90 is tested for use in Western Blotting, Immunocytochemistry and Immunohistochemistry (Paraffin), for the detection of NeuN. Less<<
Anti-NeuN Antibody: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
RNA binding protein fox-1 homolog 3 (UniProt: Q8BIF2; also known as Fox-1 homolog C, Hexaribonucleotide-binding protein 3, Fox-3, Neuronal nuclei antigen, NeuN antigen) is encoded by the Rbfox3 (also known as D11Bwg0517e, Hrnbp3) gene (Gene ID: 52897) in murine species. The NeuN is localized in nuclei and perinuclear cytoplasm of most of the neurons in the central nervous system of mammals. It is widely expressed in various regions of the brain, including the cerebral cortex, hippocampus, thalamus, caudate/putamen, cerebellum, as well as in the spinal cord. However, it is not detected in Cajal-Retzius cells in the neocortex, Purkinje cells, and photoreceptor cells. NeuN expression has been reported as early as embryonic day 9.5 (E9.5) and by E12.5 it can be found in the developing ventral horns and is also detected in the developing dorsal horns and in the dorsal root ganglion. It is expressed from embryonic stage to adulthood. Its expression is enhanced by retinoic acid treatment. It can serve as a pre-mRNA alternative splicing regulator and regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay. Anti-NeuN antibodies can identify most types of neurons in the whole nervous system with some rare exceptions. Antibodies for NeuN predominantly associate with cell nuclei and, to a lesser extent, with the perinuclear cytoplasm. Two isoforms of the NeuN protein (46 and 48 kDa) are present in both locations but differ in their relative concentration in the nucleus and cytoplasm.NeuN knockout mice display significantly reduced brain weight, impaired neurofilament expression and decreased white matter volume. They show increased susceptibility to seizures and reduced anxiety-related behaviors compared with wild type littermates. (Ref.: Lin, YS et al. (2018). PLOS One. 13(2); e0192355; Mao, S., et al. (2016). Front. Neuroanat. 13;10:54).
References
Product Information
Format
Serum
HS Code
3002 15 90
Control
Mouse brain E16 tissue lysate
Presentation
Guinea Pig polyclonal antibody in serum with 0.05% sodium azide.
This guinea pig polyclonal Anti-NeuN, Cat. No. ABN90 is tested for use in Western Blotting, Immunocytochemistry and Immunohistochemistry (Paraffin), for the detection of NeuN.
Key Applications
Western Blotting
Immunohistochemistry (Paraffin)
Immunocytochemistry
Application Notes
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:5,000 dilution from a representative lot detected NeuN in mouse cerebral cortex and hippocampus brain tissue sections.
Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected NeuN in rat E18 cortical cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
Immunogen
GST-tagged recombinant fragment corresponding to the first 97 amino acids of mouse NeuN.
Epitope
N-terminus
Host
Guinea Pig
Specificity
This Guinea pig polyclonal antibody detects NeuN. It targets an epitope within the N-terminal region.
Species Reactivity
Mouse
Species Reactivity Note
Mouse.
Antibody Type
Polyclonal Antibody
Gene Symbol
Rbfox3
Purification Method
Unpurified
Molecular Weight
Target molecular weight ~48 kDa observed; 40.61 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in Mouse E16 brain tissue lysate.
Western Blotting Analysis: A 1:2,000 Dilution of this antibody detected NeuN in Mouse E16 brain tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Neurovascular interactions are essential for proper brain function. While the effect of neural activity on cerebral blood flow has been extensively studied, whether or not neural activity influences vascular patterning remains elusive. Here, we demonstrate that neural activity promotes the formation of vascular networks in the early postnatal mouse barrel cortex. Using a combination of genetics, imaging, and computational tools to allow simultaneous analysis of neuronal and vascular components, we found that vascular density and branching were decreased in the barrel cortex when sensory input was reduced by either a complete deafferentation, a genetic impairment of neurotransmitter release at thalamocortical synapses, or a selective reduction of sensory-related neural activity by whisker plucking. In contrast, enhancement of neural activity by whisker stimulation led to an increase in vascular density and branching. The finding that neural activity is necessary and sufficient to trigger alterations of vascular networks reveals an important feature of neurovascular interactions.
