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Wählen Sie konfigurierbare Panels & Premixed-Kits - ODER - Kits für die zelluläre Signaltransduktion & MAPmates™
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Wählen Sie gebrauchsfertige Kits zur Erforschung gesamter Signalwege oder Prozesse. Oder konfigurieren Sie Ihre eigenen Kits mit Singleplex MAPmates™.
Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
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96-Well Plate
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48-602MAG
Buffer Detection Kit for Magnetic Beads
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Anti-NKp30/CD337, clone F252, Cat. No. MABF3113. is a mouse monoclonal antibody that detects -NKp30/CD337 and is tested for use in Flow Cytometry, Neutralizing, and Inhibition.
More>>Anti-NKp30/CD337, clone F252, Cat. No. MABF3113. is a mouse monoclonal antibody that detects -NKp30/CD337 and is tested for use in Flow Cytometry, Neutralizing, and Inhibition. Less<<
Natural cytotoxicity triggering receptor 3 (UniProt: O14931; also known as Activating natural killer receptor p30, Natural killer cell p30-related protein, NK-p30, NKp30, CD337) is encoded by the NCR3 (also known as 1C7, LY117) gene (Gene ID: 259197) in human. NKp30 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 19-135), a transmembrane domain (aa 136-156), and a cytoplasmic domain (aa 157-201). It is selectively expressed by all resting and activated NK cells. Its weaker expression is observed in spleen. It serves as a receptor of NK cells. It is activated by binding of extracellular ligands including BAG6 and NCR3LG1 and stimulates NK cells cytotoxicity toward neighboring cells producing these ligands. It cooperates with NKp46 and/or NKp44 in inducing NK-mediated cytotoxicity against the majority of target cells. It is also reported to control NK cells cytotoxicity against tumor cells. Its interaction with BAG6 promotes myeloid dendritic cells (DC) maturation, both through killing DCs that fail to achieve a mature phenotype and by inducing the release of TNF- and interferon- by NK cells that promote DC maturation. Masking of NKp30 with specific monoclonal antibodies is reported to reduce maturation of DCs cocultured with NK cells. Addition of supernatant from NK cells stimulated via NKp30 is shown to induce DC maturation. Six isoforms of NKp30 have been described that are produced by alternative splicing. (Ref.: Vitale, M., et al. (2005). Blood. 106(2); 566-571; Pende, D., et al. (1999). J. Exp. Med. 190(10); 1505-1516).
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgM in PBS without preservatives.
Anti-NKp30/CD337, clone F252, Cat. No. MABF3113. is a mouse monoclonal antibody that detects -NKp30/CD337 and is tested for use in Flow Cytometry, Neutralizing, and Inhibition.
Key Applications
Flow Cytometry
Affects Function
Neutralizing
Inhibition
Application Notes
Tested Applications
Flow Cytometry Analysis: A representative lot detected NKp30 (CD337) in Flow Cytometry application. (Meazza, R., et al. (2020). Cancers (Basel). 12(8):2187; Salome, B., et al. (2022). Cancer Cell. 40(9):1027-1043.e9).
Inhibition: A representative lot Inhibited the lysis of antigen presenting cells (APC) and blocked the expression of CD83, CD86.(Spaggiari, G.M., et al. (2001). Eur J Immunol.;31(6):1656-65; Vitale, M., et al. (2005). Blood. 106(2):566-71).
Flow Cytometry Analysis: A representative lot detected NKp30 (CD337) in Human peripheral blood mononuclear cells (PBMC). (Courtesy of Dr. Raffaella Meazza, IRCCS Ospedale Policlinico San Martino, Genova, Italy).
Neutralizing: A representative lot neutralized the receptor function of NKp30 (CD337). (Witkowski, M., et al. (2021). Nature. 600(7888):295-301).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
Immunogen
Activated human natural killer (NK) cells.
Epitope
Extracellular domain
Clone
F252
Concentration
1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Host
Mouse
Specificity
Clone F252 is a mouse monoclonal antibody that detects Natural cytotoxicity triggering receptor 3 (NKp30/CD337). It targets an epitope within the extracellular domain.
Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).
Flow Cytometry Analysis: 1 µg of this antibody detected NKp30 in one million Human peripheral blood mononuclear cells (PBMC).
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
