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48-602MAG
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07-2195
Sigma-AldrichAnti-NDRG1 Antibody
Detect the NDRG1 protein using this Anti-NDRG1 validated for use in WB.
More>>Detect the NDRG1 protein using this Anti-NDRG1 validated for use in WB. Less<<
Anti-NDRG1 Antibody: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Reducing agents and tunicamycin-responsive protein
protein NDRG1
N-myc downstream-regulated gene 1 protein
Background Information
N-myc down-regulated gene 1 (NDRG1) is a member of the NDRG family, which is a part of the alpha/beta hydrolase superfamily. It is mostly found in the cytoplasm, but at times migrates to the cell membrane and adherens junctions. NDRG1 is up-regulated during hypoxia and this may infer a role in protection from ischemic cell damage. NDRG1 levels in certain cancers may be a useful diagnostic tool. NDRG1 is also required for p53-mediated caspase activation and apoptosis. NDRG1 overexpression may be a great indicator of tumor aggressiveness and prognosis.
References
Product Information
Format
Affinity Purified
Control
Mouse embryonic fibroblast tissue lysate
Presentation
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
Application
Detect the NDRG1 protein using this Anti-NDRG1 validated for use in WB.
Key Applications
Western Blotting
Biological Information
Immunogen
GST-tagged recombinant protein corresponding to human NDRG1.
Host
Rabbit
Species Reactivity
Mouse
Human
Species Reactivity Note
Demonstrated to react with mouse. Predicted to react with human based on 100% sequence homology. Other homologies: Rat (94% sequence homology).
This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. It is necessary for p53-mediated caspase activation and apoptosis. Mutation in this gene has been reported to be causative for hereditary motor and sensory neuropathy-Lom. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq].
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Cell membrane. Note: Whereas in prostate epithelium and placental chorion it is located in both the cytoplasm and the nucleus, nuclear staining is not observed in colon epithelium cells. Instead its localization changes from the cytoplasm to the plasma membrane during differentiation of colon carcinoma cell lines in vitro.
TISSUE SPECIFICTY: Ubiquitous; expressed most prominently in placental membranes and prostate, kidney, small intestine, and ovary tissues. Reduced expression in adenocarcinomas compared to normal tissues. In colon, prostate and placental membranes, the cells that border the lumen show the highest expression.
INDUCTION: By homocysteine, 2-mercaptoethanol, tunicamycin in endothelial cells. Induced approximately 20-fold during in vitro differentiation of the colon carcinoma cell lines HT29-D4 and Caco-2. Induced by nickel compounds in all tested cell lines. The primary signal for its induction is an elevation of free intracellular calcium ion caused by nickel ion exposure. Okadaic acid, a serine/threonine phosphatase inhibitor, induced its expression more rapidly and more efficiently than nickel.
INVOLVEMENT IN DISEASE: Defects in NDRG1 are the cause of Charcot-Marie-Tooth disease type 4D (CMT4D) [MIM:601455]; also known as hereditary motor and sensory neuropathy Lom type (HMSNL). CMT4D is a recessive form of Charcot-Marie-Tooth disease, the most common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology: primary peripheral demyelinating neuropathy and primary peripheral axonal neuropathy. Demyelinating CMT neuropathies are characterized by severely reduced nerve conduction velocities (less than 38 m/sec), segmental demyelination and remyelination with onion bulb formations on nerve biopsy, slowly progressive distal muscle atrophy and weakness, absent deep tendon reflexes, and hollow feet. By convention, autosomal recessive forms of demyelinating Charcot-Marie-Tooth disease are designated CMT4.
SEQUENCE SIMILARITIES: Belongs to the NDRG family.
Molecular Weight
~46 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blot in mouse embryonic fibroblast tissue lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody detected NDRG1 on 10 µg of mouse embryonic fibroblast tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
