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MABN1545 Anti-MRP2 Antibody, clone 4C9.2

Anti-MRP2, clone 4C9.2, Cat. No. MABN1545, is a highly specific mouse monoclonal antibody, that targets ABCC2 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-MRP2 Antibody, clone 4C9.2: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Neuroscience Gene Symbol: ABCC2, CMOAT, CMOAT1, CMRP, DJS, MRP2 UniProt Number: Q92887
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MABN1545
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN1545
      DescriptionAnti-MRP2 Antibody, clone 4C9.2
      Alternate Names
      • Canalicular multispecific organic anion transporter 1
      • ATP-binding cassette sub-family C member 2
      • Canalicular multidrug resistance protein
      • cMRP
      • Multidrug resistance-associated protein 2
      Background InformationCanalicular multispecific organic anion transporter 1 (UniProt Q92887; also known as ATP-binding cassette sub-family C member 2, Canalicular multidrug resistance protein, cMRP, Multidrug resistance-associated protein 2) is encoded by the ABCC2 (also known as CMOAT, CMOAT1, CMRP, DJS, MRP2) gene (Gene ID 1244) in human. MRP2/ABCC2 belongs to the family of human ATP-binding cassette (ABC) transporters that are classified into seven subfamilies from ABC-A to ABC-G based on their sequence similarities. P-glycoprotein (P-gp/ABCB1), MRP2/ABCC2, and breast cancer resistance protein (BCRP/ABCG2) are well known plasma membrane ABC transporters that mediate the export of intracellular drugs, drug conjugates and metabolites. MRP2/ABCC2 is the second member of the ABCC subfamily of 13 transporters (ABCC1 to ABCC13). MRP2 is expressed in the apical hepatocyte plasma membrane, renal proximal tubules and small intestine, where it plays a role in the elimination and oral bioavailability of drugs, xenotoxins and their metabolites. MRP2 primarily functions as an organic anion transporter to mediate the elimination of bile acids, GSH and conjugated metabolites of numerous drugs and other xenobiotics. Weakly basic drugs appear to be co-transported with GSH by MRP2. Methotrexate, anthracyclines (doxorubicin, epirubicin), mitoxantrone, cisplatin, and etoposide are well known chemotherapeutic substrates of MRP2. MRP2 contains 17-transmembrane helices (a.a. 28-48, 69-89, 94-114, 127-147, 166-186, 314-334, 361-381, 438-458, 462-482, 545-565, 588-608, 972-992, 1034-1054, 1098-1118, 1120-1140, 1212-1232, 1235-1255), having its N-terminal end (a.a. 1-27) exposed extracellularly and C-terminal end (a.a. 1256-1545) at the cytoplasmic side. Two conserved nucleotide-binding domains (NBDs; a.a. 671-678 and 1334-1341) mediate the binding and hydrolyzing ATP via an ATPase, thereby providing energy for substrates export from the cytoplasm to the extracellular space.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-MRP2, clone 4C9.2, Cat. No. MABN1545, is a highly specific mouse monoclonal antibody, that targets ABCC2 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected MRP2 in human liver tissue sections.
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to the third cytoplasmic domain of human MRP2.
      Clone4C9.2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 4C9.2 targets an epitope within the third cytoplasmic domain of human MRP2.
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ABCC2
      • CMOAT
      • CMOAT1
      • CMRP
      • DJS
      • MRP2
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~175-260 kDa observed. 174.2 kDa calculated. Larger-than-calculated sizes and smeary banding pattern are consistent with the detection of differentially glycosylated MRP2 species. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HepG2 cell lysate.

      Western Blotting Analysis: 0.1 µg/mL of this antibody detected MRP2 in 10 µg of HepG2 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABN1545 04054839056307