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MABS2000 Anti-LXR alpha Antibody, clone 2B7

Anti-LXR alpha, clone 2B7, Cat. No. MABS2000, is a mouse monocloanl antibody that detects Oxysterols receptor LXR-alpha and has been tested for use in Immunoprecipitation and Western Blotting.

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MABS2000
100 μg  
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      Übersicht

      Replacement Information
      Description
      Catalogue NumberMABS2000
      DescriptionAnti-LXR alpha Antibody, clone 2B7
      Alternate Names
      • Oxysterols receptor LXR-alpha
      • Liver X receptor alpha
      • Nuclear receptor subfamily 1 group H member 3
      • RLD-1
      Background InformationOxysterols receptor LXR-alpha (UniProt: Q62685; also known as Liver X receptor alpha, Nuclear receptor subfamily 1 group H member 3, RLD-1) is encoded by the Nr1h3 (also known as Lxra) gene in rat. Liver X receptors (LXRs) are members of the nuclear receptor superfamily that have been implicated in lipid homeostasis and glucose metabolism. Oxysterols, oxidized derivatives of cholesterol, are ligands for LXRs. LXRs act as cholesterol sensors; when cellular oxysterols accumulate as a result of increasing concentrations of cholesterol, LXR induces the transcription of genes that protect cells from cholesterol overload. LXRs bind to consensus elements (LXR response elements, LXREs) as heterodimers with isoforms of the retinoid X receptor (RXR). LXR-alpha is an important regulator of cholesterol and bile acid metabolism. It interacts with RXR and shifts RXR from its role as a silent DNA-binding partner to an active ligand-binding subunit in mediating retinoid responses through target genes defined by LXRES. In adults it is expressed in spleen, pituitary, lung, liver, and fat and a weaker expression is observed in several other tissues. LXR-alpha contains a transactivation domain (AF-1; aa 1-94) that is required for ligand-independent transactivation function; another transactivation domain (AF-2; aa 203-445) that is required for ligand-dependent transactivation function; and a ligand binding domain (aa 213-432). (Ref.: Zhao, C., and Dahlman-Wright, K. (2010). J. Endocrinol. 204, 233-240).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-LXR alpha, clone 2B7, Cat. No. MABS2000, is a mouse monocloanl antibody that detects Oxysterols receptor LXR-alpha and has been tested for use in Immunoprecipitation and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: A representative lot detected LXR alpha in Immunoprecipitation applications (Tian, J., et. al. (2016). Proc Natl Acad Sci USA. 113(29):8182-7).

      Western Blotting Analysis: A representative lot detected LXR alpha in Western Blotting applications (Tian, J., et. al. (2016). Proc Natl Acad Sci USA. 113(29):8182-7).
      Biological Information
      ImmunogenGST-tagged full length recombinant rat LXR-alpha protein.
      Clone2B7
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 2B7 detects LXR-alpha in mouse and rat liver tissue.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Rat
      Species Reactivity NoteMouse, Rat.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Nr1h3
      • Lxra
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~50 kDa observed; 50.55 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse liver tissue lysate.

      Western Blotting Analysis: 1 ug/mL of this antibody detected LXR alpha in 10 µg of mouse liver tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABS2000 04054839253089

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