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Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
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96-Well Plate
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48-602MAG
Buffer Detection Kit for Magnetic Beads
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Anti-LST1 antibody, clone 7E2 detects level of LST1 & has been published & validated for use in LST1.
More>>Anti-LST1 antibody, clone 7E2 detects level of LST1 & has been published & validated for use in LST1. Less<<
Anti-LST1, clone 7E2 Antibody: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
LST1, also known as Leukocyte-specific transcript 1 protein, Protein B144, and encoded by the gene LST1/B144, is an interesting protein expressed in many tissues in both the developing fetus as well as the adult. Over-expression induces morphological changes in many cells types that include the production of filopodia and microspikes. LST1 is also involved in dendritic cell maturation and also has anti-proliferation activity on lymphocyte proliferation. LST1 is localized to internal and Golgi membranes and is primarily a single-pass membrane bound protein. Recent research also demonstrates that LST1 is important for the assembly of tunneling nanotubes, a recently discovered new mechanism of cell-cell communication. LST1 induces nanotube formation by recruiting the small GTPase RalA to the plasma membrane and promoting its interaction with the exocyst complex. Furthermore, LST1 recruits the actin-crosslinking protein filamin to the plasma membrane and interacts with M-Sec, myosin and myoferlin, and this suggests a molecular model for nanotube generation and LST1’s essential role in their formation and maintenance.
References
Product Information
Format
Purified
Presentation
Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Anti-LST1 antibody, clone 7E2 detects level of LST1 & has been published & validated for use in LST1.
Key Applications
Western Blotting
Application Notes
Western Blotting Analysis: 1.0 µg/mL from a representative lot detected LST1 in 10 µg of human brain, human lung, human liver, human kidney, human skin, human spleen, human thymus, human pancreas, human ovary, human testis, human small intestine, human parathyroid, and human adrenal gland tissue lysate. Western Blotting Analysis: A representative lot detected LST1 in HeLa cell lysate (Schiller, C., et al. (2009). Hybridoma. 28(4):281-286).
Biological Information
Immunogen
His-tagged recombinant protein corresponding to human LST1.
~26 kDa observed. The target is universal expressed, not leukocytes specific LST1 forms dimers through disulfide bridges.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in human heart tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected LST1 in 10 µg of human heart tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Carefully orchestrated intercellular communication is an essential prerequisite for the development of multicellular organisms. In recent years, tunneling nanotubes (TNT) have emerged as a novel and widespread mechanism of cell-cell communication. However, the molecular basis of their formation is still poorly understood. In the present study we report that the transmembrane MHC class III protein leukocyte specific transcript 1 (LST1) induces the formation of functional nanotubes and is required for endogenous nanotube generation. Mechanistically, we found that LST1 induces nanotube formation by recruiting the small GTPase RalA to the plasma membrane and promoting its interaction with the exocyst complex. Furthermore, we determined that LST1 recruits the actin-crosslinking protein filamin to the plasma membrane and interacts with M-Sec, myosin and myoferlin. These results allow us to suggest a molecular model for nanotube generation. In this proposal LST1 functions as a membrane scaffold mediating the assembly of a multimolecular complex, which controls the formation of functional nanotubes.
The LST1 gene is located in the human MHC class III region and encodes transmembrane and soluble isoforms that have been suggested to play a role in the regulation of the immune response and are associated with inflammatory diseases such as rheumatoid arthritis. Here we describe the generation and characterization of the first monoclonal antibodies against LST1. Two hybridoma lines secreting monoclonal antibodies designated 7E2 and 8D12 were established. The 7E2 antibody detects recombinant and endogenous LST1 by Western blot analysis while 8D12 reacts with recombinant and endogenous LST1 in immunoprecipitation and flow cytometry procedures. The newly established antibodies were used to survey LST1 protein expression in human cell lines, which was found to be tightly regulated, allowing the expression of transmembrane isoforms but suppressing soluble isoforms.
