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AB15504 Anti-Klotho Antibody

Detect Klotho using this Anti-Klotho Antibody validated for use in ELISA & WB.

Anti-Klotho Antibody: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Neuroscience Research Sub-Category: Neurodegenerative Diseases Gene Symbol: KL, klotho, EC 3.2.1.31 [Contains: Klotho peptide]. UniProt Number: Q9UEF7
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AB15504
50 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MELISA, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB15504
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Klotho Antibody
      Background InformationAnimal models of human aging, which display the characteristic diseases associated with aging, provide insight into the cause of such diseases. Recently a transgenic mouse model, called Klotho, with several premature aging phenotypes has been described. They display premature aging phenotypes such as Osteoporosis, age related skin changes, ectopic calcifications, atrophy of genital organs and thymus, emphysema and short life span (Kuro-o et al., 1997). KL protein associated with the Klotho mutation is a 1014 amino acid long peptide. It has a putative signal sequence at its N-terminus and a single transmembrane domain near its C-terminus, which is postulated to anchor it to the membrane (Martin & Mian, 1997). The extracellular domain has two internal repeats, KL-1 and KL-2 which have 20-40% sequence identity to bacterial b-glucosidase of plants and animals and mammalian lactase glycosylceramidase suggesting a role for Klotho in sphingolipid metabolism (Martin & Mian, 1997). kl gene expression was observed to be tissue specific. Improvement of systemic aging phenotypes in kl/kl mice occurs even when the exogenous expression was limited to some organs, suggesting that KL associated aging is regulated through a humoral signaling pathway. KL has been reported to be localized on the cell surface when expressed on Cho cells (Kuro-o et al., 1997). Human kl cDNA is expected to encode a protein of 1012 amino acids and has 86% homology to mouse kl.
      References
      Product Information
      FormatAffinity Purified
      PresentationLiquid in PBS with 0.1% BSA.
      Quality LevelMQ100
      Applications
      ApplicationDetect Klotho using this Anti-Klotho Antibody validated for use in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      Application NotesWestern blot: 1-10 µg/mL using ECL.
      Immunohistochemistry: Not tested. It is recommended that the antibody be tried at 2-20 µg/mL
      ELISA: 1:10,000-1:100,000 using 50-100 ng of control peptide/well.
      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenSynthetic peptide near the C-terminus of mouse Klotho.
      HostRabbit
      SpecificityKlotho
      Species Reactivity
      • Mouse
      Species Reactivity NoteOther species have not yet been tested. The immunogen sequence is 82% conserved in human and 76% in rat klotho.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss.
      Gene Symbol
      • KL
      • klotho
      • EC 3.2.1.31 [Contains: Klotho peptide].
      Purification MethodImmunoAffinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9UEF7 # The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling (By similarity).
      SIZE: 1012 amino acids; 116181 Da
      SUBUNIT: Homodimer (By similarity).
      SUBCELLULAR LOCATION: Isoform 1: Cell membrane; Single-pass type I membrane protein. Note=Isoform 1 shedding leads to a soluble peptide. & Isoform 2: Secreted.
      TISSUE SPECIFICITY: Present in cortical renal tubules (at protein level). Soluble peptide is present in serum and cerebrospinal fluid. Expressed in kidney, placenta, small intestine and prostate. Down-regulated in renal cell carcinomas, hepatocellular carcinomas, and in chronic renal failure kidney.
      DOMAIN:SwissProt: Q9UEF7 Contains 2 glycosyl hydrolase 1 regions. However, the first region lacks the essential Glu active site residue at position 239, and the second one lacks the essential Glu active site residue at position 872.
      PTM: N-glycosylated (By similarity).DISEASE:SwissProt: Q9UEF7 # Defects in KL may be a cause of chronic renal failure complications.
      SIMILARITY: Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      AB15504 04053252327292

      Documentation

      Anti-Klotho Antibody SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Anti-Klotho Antibody Analysenzertifikate

      TitelChargennummer
      RABBIT ANTI-KLOTHO 2487600
      RABBIT ANTI-KLOTHO 2471886
      RABBIT ANTI-KLOTHO AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2429664 2429664
      RABBIT ANTI-KLOTHO AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2437304 2437304
      RABBIT ANTI-KLOTHO AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2468122 2468122
      RABBIT ANTI-KLOTHO - 3222655 3222655
      RABBIT ANTI-KLOTHO -2766397 2766397
      RABBIT ANTI-KLOTHO AFFINITY PURIFIED 2826996
      RABBIT ANTI-KLOTHO AFFINITY PURIFIED POLYCLONAL ANTIBODY 2942569
      RABBIT ANTI-KLOTHO AFFINITY PURIFIED POLYCLONAL ANTIBODY -
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      Literatur

      ÜbersichtPub Med ID
      Ageing. New mice for old questions
      Martin, G M and Mian, I S
      Nature, 390:18-9 (1997)  1997

      		9363884 9363884
      Mutation of the mouse klotho gene leads to a syndrome resembling ageing
      Kuro-o, M, et al
      Nature, 390:45-51 (1997)  1997

      		9363890 9363890