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Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
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96-Well Plate
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48-602MAG
Buffer Detection Kit for Magnetic Beads
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ABE2860-25UL
Sigma-AldrichAnti-KLF4
Anti-KLF4, Cat. No. ABE2860, is a highly specific rabbit polyclonal antibody that targets Krueppel-like factor 4 (Klf4) and has been tested for use in ChIP-seq, Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, and Western Blotting.
More>>Anti-KLF4, Cat. No. ABE2860, is a highly specific rabbit polyclonal antibody that targets Krueppel-like factor 4 (Klf4) and has been tested for use in ChIP-seq, Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, and Western Blotting. Less<<
Krueppel-like factor 4 (UniProt: Q60793; also known as Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor, KLF4) is encoded by the Klf4 (also known as Ezf, Gklf, Zie) gene (Gene ID: 16600) in murine species. Klf4 is a member of the krueppel C2H2-type zinc-finger protein family that regulates multiple biological processes, including proliferation, differentiation, development, and apoptosis. KLF4 is a developmentally regulated transcription factor that plays an important role in maintaining the pluripotent state in embryonic stem cells (ESCs) and can be used as a marker of the pluripotent state for cells in culture. It can serve both as activator and as repressor depending upon its environment, but its most prominent function is growth suppression. Interestingly, increased expression of Klf4 leads to an increase in expression of P21WAF1/CIP1, which is an important checkpoint protein that inhibits cell cycle progression and is essential in mediating the cell cycle arrest at both the G1/S and G2/M boundaries. Klf4 also mediates the normal functions of the tumor suppressor p53 and contributes to the down-regulation of p53/TP53 transcription. Mutations in Klf4 gene can lead to unchecked growth and tumor formation. KLF4 is shown to be often mutated in many colon cancer cell lines and the lack of Klf4 expression is correlated with colorectal cancer pathogenesis. Elevated expression of Klf4 gene has been linked to gastric cancer, breast cancer, and squamous cell carcinomas. KLF4 is reported to target the primary miR1-2 loop promoter and stimulates miR-1 expression. Loss of KLF4 expression is considered as a mechanistic link between aggressive prostate cancer progression and low canonical AR output through miR-1 inactivation. (Ref.: Jiang, J., et al. (2008). Nat. Cell Biol. 10(3); 353-360; Siu, MK et al. (2016). Oncogenesis 5: e282).
References
Product Information
Format
Serum
Presentation
Rabbit polyclonal antiserum with 0.05% sodium azide.
Applications
Application
Anti-KLF4, Cat. No. ABE2860, is a highly specific rabbit polyclonal antibody that targets Krueppel-like factor 4 (Klf4) and has been tested for use in ChIP-seq, Chromatin Immunoprecipitation (ChIP), Immunoprecipitation, and Western Blotting.
Key Applications
ChIP-seq
Chromatin Immunoprecipitation (ChIP)
Immunoprecipitation
Western Blotting
Application Notes
Immunoprecipitation Analysis: A representative lot immunoprecipitated KLF4 in Immunoprecipitation applications (Jiang, J., et. al. (2008). Nat Cell Biol. 10(3):353-60).
Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected KLF4 in Chromatin Immunoprecipitation applications (Edupuganti, R.R., et. al. (2017). Stem Cell Reports. 9(4):1291-1303).
Western Blotting Analysis: A representative lot detected KLF4 in Western Blotting applications (Chen, X., et. al. (2008). Cell. 133(6):1106-17).
ChIP-seq Analysis: A representative lot detected KLF4 in ChIP-seq applications (Chen, X., et. al. (2008). Cell. 133(6):1106-17).
Biological Information
Immunogen
GST/His-tagged recombinant fragment corresponding to 320 amino acids from the N-terminal and internal region of murine Klf4.
Epitope
unknown
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Specificity
This rabbit polyclonal antibody detects Klf4 in mouse embryonic stem cells.
~55 kDa observed; 51.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in mouse embryonic stem cells.
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected KLF4 in lysate from mouse emmbryonic stem cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
