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ABT1366-25UG Anti-KIF18A

Anti-KIF18A , Cat. No. ABT1366, is a rabbit polyclonal antibody that detects KIF18A and is tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

NeuABT1366-25UG
25 μg  
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      Übersicht

      Replacement Information
      Description
      Catalogue NumberABT1366-25UG
      DescriptionAnti-KIF18A
      Alternate Names
      • Kinesin-like protein KIF18A
      • Marrow stromal KIF18A
      • MS-KIF18A
      Background InformationKinesin-like protein KIF18A (UniProt: Q8NI77; also known as Marrow stromal KIF18A, MS-KIF18A) is encoded by the KIF18A (also known as OK/SW-cl.108) gene (Gene ID: 81930) in human. KIF18A is a widely expressed member of the kinesin superfamily of microtubule-associated molecular motors that hydrolyze ATP to produce force and movement along microtubules. Its kinesin motor domain is localized in amino acids 11-355. It is essential in aligning mitotic chromosomes at the spindle equator during cell division. It plays a role in chromosome congression by reducing the amplitude of pre-anaphase oscillations and slows poleward movement during anaphase, leading to suppression of chromosome movements. It preferentially concentrates on microtubules (MT) plus ends attached to a lagging kinetochore. It possesses both plus-end-directed motility and microtubule depolymerizing activity. It is reported to depolymerize longer microtubules more quickly than shorter ones. The depletion of KIF18A is reported to induce aberrantly long mitotic spindles and loss of tension across sister kinetochores, which results in the activation of the Mad2-dependent spindle-assembly checkpoint. Overexpression of KIF18A is reported in several cancers and is linked to poor overall survival. It is shown to accelerate the invasion and migration of head and neck squamous cell carcinoma. (Ref.: Liu, T., et al. (2023). Biomolecules. 13(2); 326; Liu, G., et al. (2019). Transl. Cancer Res. 8(6); 2252-2263; Du, Y., et al. (2010). Current Biology 20(4); 374-380).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-KIF18A , Cat. No. ABT1366, is a rabbit polyclonal antibody that detects KIF18A and is tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      Application NotesTested Applications

      Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected Human KIF18A in human colon tissue sections.

      Immunofluorescence Analysis: A representative lot detected Human KIF18A in Immunofluorescence applications.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of human Kinesin-like protein KIF18A..
      EpitopeC-terminal
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detects Kinesin-like protein KIF18A. It targets an epitope within 20 amino acids from the C-terminal region.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • KIF18A
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~110 kDa observed; 102.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293T cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected KIF18A in HEK293T cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ABT1366-25UG 04065271059115