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MAB2008 Anti-Integrin β3 Antibody, clone SAP

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MAB2008
100 µg  
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      Übersicht

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHC, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB2008
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Integrin β3 Antibody, clone SAP
      Alternate Names
      • CD61
      Product Information
      FormatPurified
      PresentationPurified immunoglobulin. Liquid at 1 mg/mL in 0.02M phosphate buffer, 0.25M sodium chloride, pH 7.6, containing 0.1% sodium azide as a preservative.
      Quality LevelMQ100
      Applications
      ApplicationDetect Integrin β3 using this Anti-Integrin β3 Antibody, clone SAP validated for use in WB, IH, IH(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry (frozen and fixed tissue sections) including paraffin embedded sections. {See Van Belle et al 1999, original paper detailing the use in human, paraffin tissues}

      http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10333228.

      Western blot: recognizes ~115kDa band in reduced

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenMembranes of freshly isolated human platelets.
      CloneSAP
      HostMouse
      SpecificityMonoclonal antibody MAB2008 is specific for the beta-3 subunit of the vitronectin receptor (VNR)J, which is expressed on endothelial cells, platelets, melanoma, and glioma cells. The antibody is of high affinity.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe ITGB3 protein product is the integrin beta chain beta 3. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. Integrin beta 3 is found along with the alpha IIb chain in platelets. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.
      Gene Symbol
      • ITGB3
      • GP3A
      • CD61
      • GPIIIa
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P05106 # Integrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha- IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
      SIZE: 788 amino acids; 87058 Da
      SUBUNIT: Heterodimer of an alpha and a beta subunit. Beta-3 associates with either alpha-IIb or alpha-V. Isoform Beta-3C interacts with FLNB. Interacts with HIV-1 Tat.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Isoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.
      PTM: Phosphorylated on tyrosine residues in response to thrombin- induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. SHC binding also occurs when Tyr-785 alone is phosphorylated.
      DISEASE: SwissProt: P05106 # Defects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. Its inheritance is autosomal recessive. It is characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb-IIIa complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the GPIIb-IIIa complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT variants have normal or near normal (60-100%) expression of dysfunctional receptors.
      SIMILARITY: SwissProt: P05106 ## Belongs to the integrin beta chain family. & Contains 1 VWFA domain.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Bestellnummer GTIN
      MAB2008 04053252663130