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Anti-IL-11 clone 12-2E-6G, Cat. No. MABF3030, is a rat monoclonal antibody that detects IL-11 and is tested for use in Immunohistochemistry and Western Blotting.
More>>Anti-IL-11 clone 12-2E-6G, Cat. No. MABF3030, is a rat monoclonal antibody that detects IL-11 and is tested for use in Immunohistochemistry and Western Blotting. Less<<
Interleukin-11 (UniProt: P47873 ; also known as IL-11) is encoded by the Il11 gene (Gene ID: 16156) in murine species. IL-11 is member of the IL-6 family of cytokines that is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to produce mature secreted protein. It is involved in a variety of biological responses, including hematopoiesis, bone development, and carcinogenesis. Its actions are mediated via interaction with IL-11 receptor that is composed of IL-11R and gp130. Binding of IL-11 to its receptor induces the activation of JAK that phosphorylates STAT3, which then translocates to the nucleus and activates transcription of various genes. IL-11 is reported to promote the proliferation of hepatocytes in response to liver damage. In mice subjected to liver injury, IL-11 is mainly produced by hepatocytes in response to reactive oxygen species (ROS) released from dying hepatocytes. Secreted IL-11 then induces STAT3 phosphorylation in adjacent healthy hepatocytes resulting in their compensatory proliferation. It has been reported that IL-11 production in dying hepatocytes depended on ERK2 that phosphorylates Fra-1 and prevents its proteasomal degradation. Fra-1 is subsequently recruited to the Il11 promoter and activates transcription. Clone 12-2E-6G (12) is suitable for detecting endogenous IL-11 in vivo by immunohistochemistry. It is shown to detect IL-11 positive cells in colon cancer in azoxymethane plus dextran sulfate treated wild-type mice but does not detect IL-11 positive cells in Il11-deficient mice or normal colonic tissue in wild-type mice. (Ref.: Deguchi, Y., et al. (2018). Biochem. Biophys. Res. Commun. 505(2); 453-459; Nishina, T., et al. (2012). Sci. Signal. 5(207); ra5).
References
Product Information
Format
Purified
Control
MilliporeSigma offers a comprehensive range of trusted and highly cited primary and secondary antibodies for your research needs. Explore our 94
000+ and ever-growing portfolio of antibodies to meet your research needs in Apoptosis and cancer
Cell structure
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and more. Visit our Antibody Explorer Tool to view antibodies by clonality
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Presentation
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Anti-IL-11 clone 12-2E-6G, Cat. No. MABF3030, is a rat monoclonal antibody that detects IL-11 and is tested for use in Immunohistochemistry and Western Blotting.
Key Applications
Western Blotting
Immunohistochemistry
Application Notes
Tested applications
Western Blotting Analysis: A representative lot detected IL-11 in Western Blotting application (Deguchi, Y., et al. (2018). Biochem Biophys Res Commun. 505(2):453-459; Nishina, T., et al. (2021). Nat Commun. 12(1):2281).
Immunohistochemistry Applications: A representative lot detected IL-11 in Immunohistochemistry application (Deguchi, Y., et al. (2018). Biochem Biophys Res Commun. 505(2):453-459; Nishina, T., et al. (2021). Nat Commun. 12(1):2281).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
Immunogen
FLAG-tagged full-length recombinant murine IL-11 produced in HEK293T cells transfected with pcDNA3-mIl11.
Epitope
Unknown
Clone
12-2E-6G
Concentration
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Host
Rat
Specificity
Clone 12-2E-6G is a rat monoclonal antibody that detects murine IL-11.
~20 kDa observed; 21.52 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in HEK293T cells transiently transfected with murine IL-11.
Western Blotting Analysis (WB): A 1:250 dilution of this antibody detected IL-11 in HEK293T cells transiently transfected with murine IL-11, but not in wild-type HEK293T cells.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
