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MAB1281B Anti-Human Nuclei Antibody, clone 235-1, Biotin Conjugate

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MAB1281B
50 µL  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICCMBiotinMonoclonal Antibody
      Description
      Catalogue NumberMAB1281B
      DescriptionAnti-Human Nuclei Antibody, clone 235-1, Biotin Conjugate
      Alternate Names
      • HuNu
      Background InformationCell replacement techniques such as stem-cell or fetal-cell therapies show great promise for many diseases. Cross-species grafting between human and animal cells or tissues requires the identification and localization of the donor material in the host environment. Antibodies to human-specific nuclei are useful cytological and histological markers for identifying human cells in xenograft models. MAB1281 stains nuclei of all human and primate cell types giving a diffuse nuclear staining pattern. Chromosomes are negatively stained in metaphase cells. The antibody does not react with nuclei from mouse or rat.
      References
      Product Information
      FormatBiotin
      Control
      • A431 human epidermoid cancer cells.
      PresentationPurified mouse monoclonal IgG1 conjugated to Biotin in PBS with 0.1% sodium azide and 15mg/ml BSA.
      Quality LevelMQ100
      Applications
      ApplicationDetect Nuclei Marker using this mouse monoclonal antibody, Anti-Human Nuclei Antibody, clone 235-1 & Biotin Conjugate validated for use in ICC.
      Key Applications
      • Immunocytochemistry
      Application NotesEvaluated by Immunocytochemistry in human bone marrow mesenchymal stem cells (SCR108). Immunocytochemistry Analysis: A 1:200 dilution of this antibody detected nuclei in human bone marrow mesenchymal stem cells (SCR108). Evaluated by Immunocytochemistry in primary mouse embryonic fibroblasts (PMEFs). Immunocytochemistry Analysis: A 1:200 dilution of this antibody did not detect nuclei in primary mouse embryonic fibroblasts (PMEFs).
      Biological Information
      ImmunogenHuman nuclei
      Clone235-1
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes human and primate nuclei. Antibody is not reactive with rat or mouse nuclei.
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G Purified
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in A431 human epidermoid cancer cells. Immunocytochemistry Analysis: A 1:200 dilution of this antibody detected nuclei in A431 human epidermoid cancer cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8ºC protected from light in undiluted aliquots for up to 6 months from date of receipt.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MAB1281B 04053252917615

      Documentation

      Anti-Human Nuclei Antibody, clone 235-1, Biotin Conjugate SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Anti-Human Nuclei Antibody, clone 235-1, Biotin Conjugate Analysenzertifikate

      TitelChargennummer
      Anti-Human Nuclei, clone 235-1, Biotin -2624978 2624978
      Anti-Human Nuclei, clone 235-1, Biotin -2759814 2759814
      Anti-Human Nuclei, clone 235-1, Biotin Conjugate 3012159
      Anti-Human Nuclei, clone 235-1, Biotin Conjugate 3113130
      Anti-Human Nuclei, clone 235-1, Biotin Conjugate 2990205
      Anti-Human Nuclei, clone 235-1, Biotin Conjugate 2897265
      Anti-Human Nuclei, clone 235-1, Biotin Conjugate - 2424803 2424803
      Anti-Human Nuclei, clone 235-1, Biotin Conjugate - 3270755 3270755
      Anti-Human Nuclei, clone 235-1, Biotin Conjugate - 3324994 3324994
      Anti-Human Nuclei, clone 235-1, Biotin Conjugate - 3420902 3420902

      Literatur

      ÜbersichtPub Med ID
      Disruption of astrocyte-vascular coupling and the blood-brain barrier by invading glioma cells.
      Watkins, S; Robel, S; Kimbrough, IF; Robert, SM; Ellis-Davies, G; Sontheimer, H
      Nature communications  5  4196  2014

      Abstract anzeigen
      24943270 24943270