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MABE1005 Anti-HACE1 Antibody, clone 3A11.1

Anti-HACE1 Antibody, clone 3A11.1 is an antibody against HACE1 for use in Western Blotting, Immunohistochemistry (Paraffin).

Anti-HACE1 Antibody, clone 3A11.1 : SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Ubiquitin & Ubiquitin Metabolism Gene Symbol: HACE1, KIAA1320 UniProt Number: Q8IYU2
MABE1005
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1005
      DescriptionAnti-HACE1 Antibody, clone 3A11.1
      Alternate Names
      • E3 ubiquitin-protein ligase HACE1
      • HACE1
      • HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1
      Background InformationE3 ubiquitin-protein ligase HACE1 (UniProt Q8IYU2; also known as HECT domain and ankyrin repeat-containing E3 ubiquitin-protein ligase 1) is encoded by the HACE1 (also known as KIAA1320) gene (Gene ID 57531) in human. HACE1 is a ubiquitously expressed HECT (Homologous to the E6-AP Carboxyl Terminus) family E3 ligase with relatively higher expression in heart, placenta, kidney, and brain. HACE1 is a 909-amino acid protein composed of six ankyrin (ANK) repeats/domains (a.a. 64-257) in its N-terminal region and a HECT domain in its C-terminal half (a.a. 574-909). HACE1 a dual function E3 ligase that mediates target cargo degradation by at least two independent mechanisms with different E3 ligase requirements. HACE1 participates in proteasomal degradation via its HECT domain E3 ligase and in autophagic degradation via its ANK domain-mediated protein–protein interactions. HACE1-mediated cellular proteostasis only becomes apparent under stress. Much of HACE1 research activities are focused on its roles in oxidative stress in brain, autophagy in the heart during haemodynamic stress, and stress-induced tumorigenesis.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-HACE1 Antibody, clone 3A11.1 is an antibody against HACE1 for use in Western Blotting, Immunohistochemistry (Paraffin).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected HACE1 in 10 µg of HEK293 cell lysate.
      Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected HACE1 in human kidney and mouse cerebral cortex tissue sections.
      Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected HACE1 in human small intestine and rat testis tissue sections.
      Biological Information
      ImmunogenGST-tagged recombinant human HACE1 internal fragment.
      EpitopeInternal.
      Clone3A11.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityExpected to react with spliced isoforms 1 and 3 (UniProt P23771-1 and P23771-3). Reactivity toward spliced isoforms 2 and 4 (UniProt P23771-2 and P23771-4) has not been tested.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HACE1
      • KIAA1320
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~103 kDa observed. 102.3 kDa (isoform 1), 36.0 kDa (isoform 2), 63.9 kDa (isoform 3), and 78.0 kDa (isoform 4) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse brain tissue lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected HACE1 in 10 µg of mouse brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABE1005 04055977314243