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AB1783 Anti-Glutamate Transporter Antibody, Glial

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AB1783
50 µL  
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      Übersicht

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIH(P), IF, WBGpSerumPolyclonal Antibody
      Description
      Catalogue NumberAB1783
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Glutamate Transporter Antibody, Glial
      Alternate Names
      • GLT-1
      • EAAT2
      Background InformationGlutamate transporters (GluT) function to remove L-glutamate (Glu), the primary excitatory neurotransmitter in the mammalian central nervous system (CNS), from the synaptic cleft. By clearing the synapse in this manner Glu can be recycled for later use, the proper diffusion gradient can be maintained and excitotoxicity can be prevented. There have been reports of many different members of a GluT multigene family; GLT1, GLAST, EAAC1 and EAAT2. mRNA has been identified in brain for each of these proteins.

      EAAT2 transports L-glutamate and also L- and D-aspartate. It is essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
      Product Information
      FormatSerum
      Control
      • Rat brain tissue
      PresentationGuinea pig antiserum containing no preservatives.
      Quality LevelMQ100
      Applications
      ApplicationDetect Glutamate Transporter using this Anti-Glutamate Transporter Antibody, Glial validated for use in IH(P), IF & WB.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunofluorescence
      • Western Blotting
      Application NotesEvaluated by Western Blot on mouse brain membrane lysates.

      Western Blot: 1:500 dilution of this antibody detected GLT-1 on 10 ug of mouse brain membrane lysates.

      Immunohistochemistry:
      1:1,000-1:4,000 dilution from a previuos lot used a DAB detection system on adult rat forebrain fixed with 4% paraformaldehyde.

      Immunohistochemistry:
      1:5,000-10,000 dilution from a previuos lot used a cyanine conjugated secondary antibody.

      Enzymatic detection requires substantially higher primary antibody dilutions. Lightly fixed 4% PFA material is recommended.

      Notes:

      Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with 50 mL of Ca2+-free Tyrode+s solution followed by a formalin-picric acid fixative (4% paraformaldehyde with 0.4% picric acid in 0.16 M phosphate buffer, pH 6.9) for 6 minutes. Tissues were rapidly dissected out, postfixed in the same fixative for 90 minutes and rinsed for at least 24 hours in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Sections were cut (14 um) in a cryostat and incubated at 4°C overnight with AB1783 (1:5,000-1:10,000). After rinsing in PBS sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.



      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenSynthetic peptide from the carboxy-terminus of rat GLT-1.
      EpitopeC-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostGuinea Pig
      SpecificityGlial Glutamate Transporter GLT-1 (EAAT2). The antibody has been tested on central nervous system tissue. Preabsorption of the antiserum with the immunogen peptide (Catalog number AG391) completely abolishes the immunostaining.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of a family of solute transporter proteins. The membrane-bound protein is the principal transporter that clears the excitatory neurotransmitter glutamate from the extracellular space at synapses in the central nervous system. Glutamate clearance is necessary for proper synaptic activation and to prevent neuronal damage from excessive activation of glutamate receptors. Mutations in and decreased expression of this protein are associated with amyotrophic lateral sclerosis. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
      Gene Symbol
      • SLC1A2
      • GLT-1
      • EAAT2
      • GLT1
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P43004 # Transports L-glutamate and also L- and D-aspartate. Essential for terminating the postsynaptic action of glutamate by rapidly removing released glutamate from the synaptic cleft. Acts as a symport by cotransporting sodium.
      SIZE: 574 amino acids; 62104 Da
      SUBUNIT: Homotrimer.
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      PTM: Glycosylated.
      SIMILARITY: SwissProt: P43004 ## Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family.
      Molecular Weight~62 kDa
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on mouse brain membrane lysates.

      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt.
      Packaging Information
      Material Size50 µL
      Global Trade Item Number
      Bestellnummer GTIN
      AB1783 04053252509391