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MABN1724 Anti-GABA(A) Receptor α2 Antibody, clone N399/19

Detect GABA(A) Receptor α2 using this Anti-GABA(A) Receptor α2 Antibody, clone N399/19 validated for use in Western Blotting, Immunohistochemistry.

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Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters Gene Symbol: Gabra2, Gabra-2 UniProt Number: P23576
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MABN1724
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, MWB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN1724
      DescriptionAnti-GABA(A) Receptor α2 Antibody, clone N399/19
      Alternate Names
      • Gamma-aminobutyric acid receptor subunit alpha-2
      • GABA(A) receptor subunit alpha-2
      • Gamma-aminobutyric acid receptor A2 subunit
      Background InformationGamma-aminobutyric acid receptor subunit alpha-2 (UniProt P23576; also known as GABA(A) receptor subunit alpha-2, Gamma-aminobutyric acid receptor A2 subunit) is encoded by the Gabra2 (also known as Gabra-2) gene (Gene ID 289606) in rat species. The GABAA receptor (GABAAR) is an ionotropic receptor and ligand-gated ion channel. Its endogenous ligand is the inhibitory neurotransmitter γ-aminobutyric acid (GABA) in the central nervous system (CNS). Upon activation, the GABAA receptor selectively conducts chloride ion through its pore, resulting in hyperpolarization of the neuron. The receptor is a pentameric complex composed of two alpha, two beta, and one gamma subunits arranged around a central pore. Each subunit contains four transmembrane domains with both the N- and C-terminus located extracellularly. In human and rat, there exist six types of alpha subunits (encoded by GABRA1-6), three types of beta subunits (encoded by GABRB1-3), and three types of gamma subunits (encoded by GABRG1-3). The alpha 2 subunit is intially produced with a signal peptide sequence (aa 1-28), which is then cleaved off to yield the mature subunit (aa 29-451) with a large N-terminal extracellular domain (a.a. 29-251), four transmembrane segments (a.a. 252-273, 279-300, 313-334, 420-441) and a large intracellular/cytoplasmic loop (a.a. 335-419) between the third and fourth transmembrane domain.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect GABA(A) Receptor α2 using this Anti-GABA(A) Receptor α2 Antibody, clone N399/19 validated for use in Western Blotting, Immunohistochemistry.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesWestern Blotting Analysis: 2.0 µg/mL from a representative lot detected GABA(A) receptor subunit alpha-2 in 10 µg rat brain tissue lysate.
      Western Blotting Analysis: A representative lot detected the GABA(A) receptor subunit alpha-2 target band only in brain membrane extracts from wild-type rats and mice, but not from Gabra2-knockout mice.
      Immunohistochemisry Analysis: A representative lot detected GABA(A) receptor subunit alpha-2 immunoreactive regions, includinghippocampus, in rat whole brain sections (Courtesy of Professor. James S. Trimmer, UC Davis, CA, USA).
      Biological Information
      ImmunogenRecombinant rat GABA(A) receptor α2 cytoplasmic fragment.
      EpitopeCytoplasmic domain.
      CloneN399/19
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone N399/19 detects the GABA(A) receptor subunit alpha-2 band only in brain membrane extracts from wild-type rats and mice, but not from Gabra2-knockout mice. Clone N399/19 does not cross-react with GABA(A) receptor subunit alpha-1.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Gabra2
      • Gabra-2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~57 kDa observed. Target band appears larger than the calculated molecular weight of 47.94 kDa due to glycosylation.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human brain tissue lysate.

      Western Blotting Analysis: 2.0 µg/mL of this antibody detected GABA(A) receptor subunit alpha-2 in 10 µg human brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABN1724 04055977329476