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Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
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Gewähltes Kit
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96-Well Plate
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
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ABF189
Sigma-AldrichAnti-FcRn/FCGRT Antibody
This Anti-FcRn/FCGRT Antibody is validated for use in Western Blotting for the detection of FcRn/FCGRT .
More>>This Anti-FcRn/FCGRT Antibody is validated for use in Western Blotting for the detection of FcRn/FCGRT . Less<<
Anti-FcRn/FCGRT Antibody : SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
IgG receptor FcRn large subunit p51 (UniProt P55899; also known as FcRn, IgG Fc fragment receptor transporter alpha chain, Neonatal Fc receptor) is encoded by the FCGRT gene (also known as FCRN) (Gene ID 2217) in human. Transfer of passive immunity from mother to the fetus or newborn involves the transport of IgG across several epithelia. Depending on the species, IgG is transported prenatally across the placenta and yolk sac or is absorbed from colostrum and milk by the small intestine of the suckling newborn. In both cases, apical to basolateral transepithelial transport of IgG is mediated by FcRn. FcRn is a heterodimer of an alpha-chain and beta(2)-microglobulin (beta(2)m) and differs from other IgG Fc receptors in that it is structurally related to MHC class I molecules. The assembly of the FcRn alpha-chain with beta(2)m is important for both cell surface expression and pH-dependent high affinity IgG binding. Proteins endocytosed from serum are degraded in the lysosomes. However, serum albumin (SA) and IgG bind to FcRn at low pH in the endosome after endocytosis, and are transported back to the cellular surface, where they are released into the bloodstream, resulting in an extended serum half-life.
References
Product Information
Format
Affinity Purified
Presentation
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
This Anti-FcRn/FCGRT Antibody is validated for use in Western Blotting for the detection of FcRn/FCGRT .
Key Applications
Western Blotting
Application Notes
Western Blotting Analysis: A representative lot detected FcRn/FCGRT in FO-1 cells expressing Myc-hFcRn (Praetor, A., et al. (2002). J Cell Sci. 115(Pt11):2389-2397). Western Blotting Analysis: A representative lot detected FcRn/FCGRT in hFcRn transfected MDCK cells (Praetor, A., et al. (1999). J Cell Sci. 112(Pt14):2291-2299).
Biological Information
Immunogen
Linear peptide corresponding to the Alpha-2/-3 domain of human FcRn/FCGRT.
Epitope
Alpha-2/-3 domain
Concentration
Please refer to lot specific datasheet.
Host
Rabbit
Species Reactivity
Human
Species Reactivity Note
Human. Predicted to react with Rat, Mouse, Feline, Canine, Monkey based on 100% sequence homology.
~35/45 kDa observed, with the lower band representing non- and/or less glycosylated form(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Western Blotting in human liver tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected FcRn/FCGRT in 10 µg of human liver tissue lysate.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
beta(2)-Microglobulin is important for cell surface expression and pH-dependent IgG binding of human FcRn. Praetor, A; Hunziker, W Journal of cell science
115
2389-97
2002
FcRn is a heterodimer of an alpha-chain and beta(2)-microglobulin (beta(2)m) and differs from other IgG Fc receptors in that it is structurally related to MHC class I molecules. Several functions attributed to FcRn are affected in beta(2)-microglobulin (beta(2)m)-deficient mice, suggesting that the alpha-chain needs to assemble with beta(2)m to form a functional receptor. However, the precise role of beta(2)m in FcRn function is not known. Here we expressed the human FcRn alpha-chain alone or in combination with beta(2)m in human melanoma FO-1 cells. We show that beta(2)m is important for cell surface expression of FcRn and that, in the absence of beta(2)m, the receptor is retained in the endoplasmic reticulum. Furthermore, in the absence of beta(2)m, IgG binding is decreased compared with that of native FcRn. Thus, assembly of the FcRn alpha-chain with beta(2)m is important for both transport of FcRn from the ER to the cell surface and efficient pH-dependent IgG binding.
Intracellular traffic of the MHC class I-like IgG Fc receptor, FcRn, expressed in epithelial MDCK cells. Praetor, A; Ellinger, I; Hunziker, W Journal of cell science
112 ( Pt 14)
2291-9
1998
Transfer of passive immunity from mother to the fetus or newborn involves the transport of IgG across several epithelia. Depending on the species, IgG is transported prenatally across the placenta and yolk sac or is absorbed from colostrum and milk by the small intestine of the suckling newborn. In both cases apical to basolateral transepithelial transport of IgG is thought to be mediated by FcRn, an IgG Fc receptor with homology to MHC class I antigens. We have now expressed the human FcRn in polarized MDCK cells and analyzed the intracellular routing of the receptor. FcRn showed a predominant intracellular localization at steady state. Newly synthesized FcRn was delivered in a non-vectorial fashion to both the apical and basolateral surfaces of MDCK cell monolayers. Following internalization from the apical or basolateral domain, the receptor transcytosed to the opposite surface. These findings provide direct evidence for the transepithelial transport function of FcRn and indicate that the receptor undergoes multiple rounds of transcytosis.
