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ABE1828 Anti-FOXK2 Antibody

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ABE1828
100 µL  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IPRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABE1828
      DescriptionAnti-FOXK2 Antibody
      Alternate Names
      • Forkhead box protein K2
      • Cellular transcription factor ILF-1
      • FOXK1
      • Interleukin enhancer-binding factor 1
      • FOXK2
      Background InformationForkhead box protein K2 (UniProt Q01167; also known as FOXK2, Cellular transcription factor ILF-1, Interleukin enhancer-binding factor 1) is encoded by the FOXK2 (also known as ILF, ILF-1, ILF1) gene (Gene ID 3607) in human. FOXK2 is a transcription factor involved in cell proliferation and survival. FOXK1 and FOXK2 belong to the family of Fox transcription factors characterized by the forkhead DNA-binding domains. However, FOXK1 and FOXK2 can be distinguished from other Fox transcription factors by the presence of the forkhead-associated (FHA) domains. FOXK2 interacts with BRCA1-associated protein 1 (BAP1) through the FHA domain in a BAP1 Thr493 phosphorylation-dependent manner. BAP1 in turn recruits HCF-1, thereby forming a ternary complex at the FOXK2 target genes sites, where BAP1 represses FOXK2 target genes expression in a BAP1 DUB activity-dependent manner.
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal serum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-FOXK2 Antibody is validated for use in Western Blotting, Immunoprecipitation for the detection of FOXK2 .
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated FOXK2 in HeLa nuclear extract (Data courtesy of El Bachir Affar, University of Montreal).
      Western Blotting Analysis: A representative lot detected FOXK2 in tag-affinity purified BAP1 complex from HeLa cells expressing Flag-HA-tagged wild-type BAP1 or catalytically dead C91S BAP1 mutant (Mashtalir, N., et al. (2014). Mol. Cell. 54(3):392-406).
      Biological Information
      ImmunogenRecombinant protein corresponding to the C-terminal region of human FOXK2.
      EpitopeC-terminal
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityExpected to react with isoforms 1&2, but not isoform 3.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • FOXK2
      • ILF
      • ILF1
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~78 kDa observed. Isoform 1 (69.1 kDa calculated); Isoform 2 (64.3 kDa calculated).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa nuclear extract.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected FOXK2 in 10 µg of HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ABE1828 04055977175301

      Documentation

      Anti-FOXK2 Antibody SDB

      Titel

      Sicherheitsdatenblatt (SDB) 

      Anti-FOXK2 Antibody Analysenzertifikate

      TitelChargennummer
      Anti-FOXK2 -Q2578149 Q2578149

      Literatur

      ÜbersichtPub Med ID
      Autodeubiquitination protects the tumor suppressor BAP1 from cytoplasmic sequestration mediated by the atypical ubiquitin ligase UBE2O.
      Mashtalir, N; Daou, S; Barbour, H; Sen, NN; Gagnon, J; Hammond-Martel, I; Dar, HH; Therrien, M; Affar, el B
      Molecular cell  54  392-406  2014

      Abstract anzeigen
      24703950 24703950

      Technische Informationen

      Titel
      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
      White Paper: Further considerations of antibody validation and usage.