Wenn Sie das Fenster schließen, wird Ihre Konfiguration nicht gespeichert, es sei denn, Sie haben Ihren Artikel in die Bestellung aufgenommen oder zu Ihren Favoriten hinzugefügt.
Klicken Sie auf OK, um das MILLIPLEX® MAP-Tool zu schließen oder auf Abbrechen, um zu Ihrer Auswahl zurückzukehren.
Wählen Sie konfigurierbare Panels & Premixed-Kits - ODER - Kits für die zelluläre Signaltransduktion & MAPmates™
Konfigurieren Sie Ihre MILLIPLEX® MAP-Kits und lassen sich den Preis anzeigen.
Konfigurierbare Panels & Premixed-Kits
Unser breites Angebot enthält Multiplex-Panels, für die Sie die Analyten auswählen können, die am besten für Ihre Anwendung geeignet sind. Unter einem separaten Register können Sie das Premixed-Cytokin-Format oder ein Singleplex-Kit wählen.
Kits für die zelluläre Signaltransduktion & MAPmates™
Wählen Sie gebrauchsfertige Kits zur Erforschung gesamter Signalwege oder Prozesse. Oder konfigurieren Sie Ihre eigenen Kits mit Singleplex MAPmates™.
Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
.
Bestellnummer
Bestellinformationen
St./Pkg.
Liste
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Wählen Sie bitte Spezies, Panelart, Kit oder Probenart
Um Ihr MILLIPLEX® MAP-Kit zu konfigurieren, wählen Sie zunächst eine Spezies, eine Panelart und/oder ein Kit.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Catalogue Number
Ordering Description
Qty/Pack
List
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Spezies
Panelart
Gewähltes Kit
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
96-Well Plate
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
Weitere Reagenzien hinzufügen (MAPmates erfordern die Verwendung eines Puffer- und Detektionskits)
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Platzsparende Option Kunden, die mehrere Kits kaufen, können ihre Multiplex-Assaykomponenten in Kunststoffbeuteln anstelle von Packungen erhalten, um eine kompaktere Lagerung zu ermöglichen.
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Das Produkt wurde in Ihre Bestellung aufgenommen
Sie können nun ein weiteres Kit konfigurieren, ein Premixed-Kit wählen, zur Kasse gehen oder das Bestell-Tool schließen.
Anti-FIP200, clone N1-8, Cat. No. MABC1748, is a mouse monoclonal antibody that detects FAK family kinase-interacting protein of 200 kDa (FIP200) and is tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.
More>>Anti-FIP200, clone N1-8, Cat. No. MABC1748, is a mouse monoclonal antibody that detects FAK family kinase-interacting protein of 200 kDa (FIP200) and is tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting. Less<<
Empfohlene Produkte
Übersicht
Replacement Information
Description
Catalogue Number
MABC1748-100UL
Description
Anti-FIP200 Antibody, clone N1-8
Alternate Names
RB1-inducible coiled-coil protein 1
FAK family kinase-interacting protein of 200 kDa
Background Information
RB1-inducible coiled-coil protein 1 (UniProt: Q8TDY2; also known as FAK family kinase-interacting protein of 200 kDa, FIP200) is encoded by the RB1CC1 (also known as KIAA0203; RBICC) gene (Gene ID: 9821) in human. FIP200 plays an important role in several biological pathways including cell proliferation and autophagy. It regulates early and late events of autophagosome formation through direct interaction with Atg16L1. FIP200 expression has been shown in cytoplasm and in the nucleus. It serves as a positive regulator of the NF-kB and mTOR signaling. It also functions as a negative factor in FAK-Erk1/2 signaling pathway. It is reported to form a complex with p53 and/or hSNF5 and the large protein complex in mammalian cells and serves as a strong activator of retinoblastoma 1 (RB1), p16, and p21 promoters. The nuclear FIP200 is shown to enhance the RB1 pathway globally and behave as a tumor suppressor in some human malignancies. Its expression levels correlate well with those of RB1 in cancer cell lines and higher expression has been shown in human musculoskeletal and cultured osteosarcoma cells. Clone N1-8 is highly suited for immunohistochemistry analysis and it recognizes an epitope within amino acids 255-271. (Ref.: Hama, Y., et al. (2012). PLoS ONE 7(3); e32052).
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Anti-FIP200, clone N1-8, Cat. No. MABC1748, is a mouse monoclonal antibody that detects FAK family kinase-interacting protein of 200 kDa (FIP200) and is tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
ELISA
Immunohistochemistry (Paraffin)
Western Blotting
Application Notes
ELISA Analysis: A representative lot detected FIP200 in ELISA applications (Hama, Y., et. al. (2012). PLoS One. 7(3):e32052).
Immunohistochemistry Applications: A representative lot detected FIP200 in Immunohistochemistry applications (Hama, Y., et. al. (2012). PLoS One. 7(3):e32052).
Western Blotting Analysis: A representative lot detected FIP200 in Western Blotting applications (Hama, Y., et. al. (2012). PLoS One. 7(3):e32052).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
Immunogen
GST-tagged recombinant fragment corresponding to 247 amino acids from the N-terminal region of human FIP200 and a C-terminal polypeptide fragment of FIP200.
Epitope
N-terminus
Clone
N1-8
Concentration
0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Host
Mouse
Specificity
Clone N1-8 is a mouse monoclonal antibody that detects Focal adhesion kinase (FAK)-interacting protein of 200 kDa (FIP200). It targets an epitope within 17 amino acids from the N-terminal half.
Evaluated by Immunohistochemistry (Paraffin) in human breast cancer tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected FIP200 in human breast cancer tissue sections.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at +2°C to +8°C from date of receipt.
RB1-inducible coiled-coil 1 (RB1CC1; also known as FIP200) plays important roles in several biological pathways such as cell proliferation and autophagy. Evaluation of RB1CC1 expression can provide useful clinical information on various cancers and neurodegenerative diseases. In order to realize the clinical applications, it is necessary to establish a stable supply of antibody and reproducible procedures for the laboratory examinations. In the present study, we have generated mouse monoclonal antibodies for RB1CC1, and four kinds of antibodies (N1-8, N1-216, N3-2, and N3-42) were found to be optimal for clinical applications such as ELISA and immunoblots and work as well as the pre-existing polyclonal antibodies. N1-8 monoclonal antibody provided the best recognition of RB1CC1 in the clinico-pathological examination of formalin-fixed paraffin-embedded tissues. These monoclonal antibodies will help to generate new opportunities in scientific examinations in biology and clinical medicine.
