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MAB3822-C Anti-Ezrin Antibody, clone 4A5, Ascites Free

Anti-Ezrin Antibody, clone 4A5, Ascites Free is an antibody against Ezrin for use in Western Blotting, Immunohistochemistry, Immunoprecipitation, Electron Microscopy, Immunocytochemistry.

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Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: EZR, VIL2 UniProt Number: Q8HZQ5
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MAB3822-C
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, RbWB, IHC, IP, EM, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB3822-C
      DescriptionAnti-Ezrin Antibody, clone 4A5, Ascites Free
      Alternate Names
      • Cytovillin
      • Villin-2
      • p81
      • Ezrin
      Background InformationEzrin (UniProt Q8HZQ5; also known as Cytovillin, Villin-2, p81) is encoded by the EZR (also known as VIL2) gene (Gene ID 100008846) in Oryctolagus cuniculus (Rabbit) species. Ezrin, radixin, and moesin are the three ERM protein family members that crosslink actin filaments with plasma membranes and play an important role in the cell cortex architecture. In its inactive state, ezrin is located in the cytosol in a conformation involving intramolecular interaction between its N- and C-terminal. Ezrin is activated by phosphorylation, which causes a conformation change that disrupts the head-to-tail intramolecular interaction. Upon activation, ezrin binds to the cytoplasmic tails of multiple adhesion and signaling membrane molecules, such as CD44, growth factor receptors, and vascular adhesion molecule (VCAM1). The initiator methionine of ezrin is removed posttranslationally to yield the mature protein (a.a. 2-586) with an N-termina FERM domain (a.a. 2-295) that mediates interaction with microtubules.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Ezrin Antibody, clone 4A5, Ascites Free is an antibody against Ezrin for use in Western Blotting, Immunohistochemistry, Immunoprecipitation, Electron Microscopy, Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunoprecipitation
      • Electron Microscopy
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: 0.5 µg/mL of this antibody from a representative lot detected Ezrin in 10 µg of HeLa cell lysate.
      Immunohistochemistry Analysis: A representative lot detected a higher Ezin immunoreactivity in paraffin-embedded human medulloblastoma sections when compared with normal cerebellum sections (Haeberle, H., et al. (2012). Neoplasia. 14(7):666-669).
      Immunohistochemistry Analysis: A representative lot immunostained parietal cells in rabbit gastric mucosa (Hanzel, D.K., et al. (1989). Am. J. Physiol. 256(6 Pt 1):G1082-G1089).
      Immunoprecipitation Analysis: A representative lot immunoprecipitated Ezin from human A431 cells. The immunoprecipitated Ezrin was phosphorylated on Ser66 from dbcAMP‐treated, but not cAMP‐treated, A431 cells (Wang, S., et al. (2009). Cell Res. 19(12):1350-1362).
      Electron Microscopy Analysis: A representative lot detected Ezrin on microvilli of the intracellular canaliculi (IC) and the subapical region of resting parietal cells in Lowicryl HM20-embedded rabbit gastric gland ultrathin sections (Sawaguchi, A., et al. (2004). J. Histochem. Cytochem. 52(1): 77–86).
      Immunocytochemistry Analysis: A representative lot detected exogenously expressed human GST fusion protein by fluorescent immunocytochemistry staining of formaldehyde-fixed, Triton X-100-permeabilized rabbit gastric parietal cells (Zhou, R., et al. (2003). J. Biol. Chem. 278(37):35651-35659).
      Western Blotting Analysis: A representative lot detected both the endogenous rabbit Ezrin as well as exogenously expressed human Ezrin GST fusion proteins in transfected rabbit gastric parietal cells (Zhou, R., et al. (2003). J. Biol. Chem. 278(37):35651-35659).
      ELISA Analysis: Clone 4A5 hybridoma culture supernatant was screened for its immunoreactivity by ELISA and found to react with both dentured and non-denatured 80 kDa Ezrin preparations from rabbit gastric glands/mucosa vesicular fractions (Hanzel, D.K., et al. (1989). Am. J. Physiol. 256(6 Pt 1):G1082-G1089).
      Biological Information
      ImmunogenPurified Ezrin from rabbit gastric parietal cells.
      Clone4A5
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 4A5 reacted with both dentured and non-denatured Ezrin (Hanzel, D.K., et al. (1989). Am. J. Physiol. 256(6 Pt 1):G1082-G1089).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Rabbit
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EZR
      • VIL2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~75 kDa observed. 69.41 kDa (Human/UniProt P15311 & Mouse/P26040), 69.39 kDa (Rat/UniProt P31977), and 69.22 kDa (Rabbit/Q8HZQ5) calculated. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in L6 rat myoblast lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Ezrin in 10 µg of L6 rat myoblast lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MAB3822-C 04055977330175