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AB5732 Sigma-AldrichAnti-En-1 Antibody

Anti-En-1 Antibody is an antibody against En-1 for use in WB, IH.

Anti-En-1 Antibody: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.

SDB
Analysenzertifikat
Datenblatt

Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience, Neuronal & Glial Markers Gene Symbol: EN1, Hu-En-1 UniProt Number: Q05925

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AB5732

100 µg

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Key Spec Table

Species Reactivity	Key Applications	Host	Format	Antibody Type
H, M	WB, IHC	Rb	Affinity Purified	Polyclonal Antibody

Description
Catalogue Number	AB5732
Brand Family	Chemicon®
Trade Name	Chemicon
Description	Anti-En-1 Antibody
Alternate Names	Engrailed

References

Product Information
Format	Affinity Purified
Presentation	Affinity purified immunoglobulin. Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
Quality Level	MQ100

Applications
Application	Anti-En-1 Antibody is an antibody against En-1 for use in WB, IH.
Key Applications	Western Blotting Immunohistochemistry
Application Notes	Western blot: 1:200-1:1,000 using ECL. Immunohistochemistry: 1:200-1:1,000. Optimal working dilutions must be determined by the end user.

Biological Information
Immunogen	Synthetic peptide from human En1.
Host	Rabbit
Specificity	Recognizes En1, (Engrailed family), a homeodomain transcription factor.
Species Reactivity	Human Mouse
Antibody Type	Polyclonal Antibody
Entrez Gene Number	NM_001426.3
Entrez Gene Summary	Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.
Gene Symbol	EN1 Hu-En-1
UniProt Number	Q05925
UniProt Summary	SIZE: 392 amino acids; 40101 Da SUBCELLULAR LOCATION: Nucleus. SIMILARITY: SwissProt: Q05925 ## Belongs to the engrailed homeobox family. & Contains 1 homeobox DNA-binding domain.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles. PREPARATION AND USE: To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL. Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses. The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C. For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality. For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

Storage and Shipping Information

Storage Conditions

Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

PREPARATION AND USE:

To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not affect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

Resuspend the antibody pellet in a suitable biological buffer such as PBS or TBS (pH 7.3-7.5) to a final concentration of 1.0 mg/mL. For example, to achieve a 1.0 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.

Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.

The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.

For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

For long-term storage at -70°C, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Bestellnummer	GTIN
AB5732	04053252740633

Documentation

Anti-En-1 Antibody SDB

Titel
Sicherheitsdatenblatt (SDB)

Anti-En-1 Antibody Analysenzertifikate

Titel	Chargennummer
RABBIT ANTI-En1 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2117126	2117126
RABBIT ANTI-En1 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2148214	2148214
RABBIT ANTI-En1 - 3321235	3321235
RABBIT ANTI-En1 - 3766725	3766725
RABBIT ANTI-En1 - 3903315	3903315
RABBIT ANTI-En1 - 4059942	4059942
RABBIT ANTI-En1 -2593912	2593912
RABBIT ANTI-En1 -2689079	2689079
RABBIT ANTI-En1 -2757922	2757922
RABBIT ANTI-En1 -2884808	2884808

Datenblatt

Titel
RABBIT ANTI-En1 AFFINITY PURIFIED POLYCLONAL ANTIBODY

Kategorien

Life Science Research > Antibodies and Assays > Primary Antibodies

Das Produkt wurde in Ihre Bestellung aufgenommen

AB5732 Sigma-AldrichAnti-En-1 Antibody

Anti-En-1 Antibody is an antibody against En-1 for use in WB, IH.

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Übersicht

Sonderaktionen

Key Spec Table

Documentation

Anti-En-1 Antibody SDB

Anti-En-1 Antibody Analysenzertifikate

Datenblatt

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