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MABT1506-25UG Anti-Desmoglein 3 Antibody, clone 5H10

Anti-Desmoglein 3, clone 5H10, Cat. No. MABT1506, is a mouse monoclonal antibody that detects Desmoglein-3 and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blotting.

Anti-Desmoglein 3 Antibody, clone 5H10: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Cell Structure Gene Symbol: DSG3, CDHF6 UniProt Number: P32926
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MABT1506-25UG
25 μg  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, ICC, IF, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT1506-25UG
      DescriptionAnti-Desmoglein 3 Antibody, clone 5H10
      Alternate Names
      • 130 kDa pemphigus vulgaris antigen
      • PVA
      • Cadherin family member 6
      Background InformationDesmoglein-3 (UniProt: P32926; also known as 130 kDa pemphigus vulgaris antigen, PVA, Cadherin family member 6) is encoded by the DSG3 (also known as CDHF6) gene (Gene ID: 1830) in human. Desmogleins are protein substances located in and on the surface of keratinocytes. They are present in specialized cell-cell adhesion structures enriched in tissues that undergo substantial mechanical stress. These proteins have been shown to be a critical factor in cell-to-cell adhesion. Desmoglein-3 is a single-pass type I membrane protein that is a component of intercellular desmosome junctions. It is involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion. It is synthesized with a signal peptide (aa 1-23) and a propeptide (aa 24-49) that are cleaved off in the mature form. It contains an extracellular domain (aa 50-615), a transmembrane domain (aa 616-640), and a cytoplasmic domain (aa 641-999). Desmoglein-3 has four cadherin domains and two desmoglein repeats. This antibody (clone 5H10) recognized the extracellular domain of desmoglein-3.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Desmoglein 3, clone 5H10, Cat. No. MABT1506, is a mouse monoclonal antibody that detects Desmoglein-3 and has been tested for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunofluorescence
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation Analysis: A representative lot detected Desmoglein 3 in Immunoprecipitation applications (Wahl, J.K. 3rd, et. al. (2002). Hybrid Hybridomics. 21(1):37-44).

      Flow Cytometry Analysis: A representative lot detected Desmoglein 3 in Flow Cytometry applications (Wan, H., et. al. (2007). Stem Cells. 25(5):1286-97).

      Western Blotting Analysis: A representative lot detected Desmoglein 3 in Western Blotting applications (Wahl, J.K. 3rd, et. al. (2002). Hybrid Hybridomics. 21(1):37-44).

      Immunocytochemistry Analysis: A representative lot detected Desmoglein 3 in Immunocytochemistry applications (Wahl, J.K. 3rd, et. al. (2002). Hybrid Hybridomics. 21(1):37-44).

      Immunofluorescence Analysis: A representative lot detected Desmoglein 3 in Immunofluorescnece applications (Wan, H., et. al. (2007). Stem Cells. 25(5):1286-97).
      Biological Information
      ImmunogenExtracellular domain of Desmoglein 3
      Epitopeextracellular domain
      Clone5H10
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 5H10 detects Desmoglein-3 in human cells. It targets an epitope with in the extracellular domain.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DSG3
      • CDHF6
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~125 kDa observed; 107.53 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HaCaT cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Desmoglein 3 in HaCaT cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABT1506-25UG 04054839531941