Wenn Sie das Fenster schließen, wird Ihre Konfiguration nicht gespeichert, es sei denn, Sie haben Ihren Artikel in die Bestellung aufgenommen oder zu Ihren Favoriten hinzugefügt.
Klicken Sie auf OK, um das MILLIPLEX® MAP-Tool zu schließen oder auf Abbrechen, um zu Ihrer Auswahl zurückzukehren.
Wählen Sie konfigurierbare Panels & Premixed-Kits - ODER - Kits für die zelluläre Signaltransduktion & MAPmates™
Konfigurieren Sie Ihre MILLIPLEX® MAP-Kits und lassen sich den Preis anzeigen.
Konfigurierbare Panels & Premixed-Kits
Unser breites Angebot enthält Multiplex-Panels, für die Sie die Analyten auswählen können, die am besten für Ihre Anwendung geeignet sind. Unter einem separaten Register können Sie das Premixed-Cytokin-Format oder ein Singleplex-Kit wählen.
Kits für die zelluläre Signaltransduktion & MAPmates™
Wählen Sie gebrauchsfertige Kits zur Erforschung gesamter Signalwege oder Prozesse. Oder konfigurieren Sie Ihre eigenen Kits mit Singleplex MAPmates™.
Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
.
Bestellnummer
Bestellinformationen
St./Pkg.
Liste
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Wählen Sie bitte Spezies, Panelart, Kit oder Probenart
Um Ihr MILLIPLEX® MAP-Kit zu konfigurieren, wählen Sie zunächst eine Spezies, eine Panelart und/oder ein Kit.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Catalogue Number
Ordering Description
Qty/Pack
List
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Spezies
Panelart
Gewähltes Kit
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
96-Well Plate
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
Weitere Reagenzien hinzufügen (MAPmates erfordern die Verwendung eines Puffer- und Detektionskits)
Menge
Bestellnummer
Bestellinformationen
St./Pkg.
Listenpreis
48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Platzsparende Option Kunden, die mehrere Kits kaufen, können ihre Multiplex-Assaykomponenten in Kunststoffbeuteln anstelle von Packungen erhalten, um eine kompaktere Lagerung zu ermöglichen.
Dieser Artikel wurde zu Ihren Favoriten hinzugefügt.
Das Produkt wurde in Ihre Bestellung aufgenommen
Sie können nun ein weiteres Kit konfigurieren, ein Premixed-Kit wählen, zur Kasse gehen oder das Bestell-Tool schließen.
Detect Desmin using this Anti-Desmin Antibody, clone DE-B-5 validated for use in WB, IH, IH(P), IH(P).
Key Applications
Immunocytochemistry
Immunohistochemistry
Immunohistochemistry (Paraffin)
Application Notes
Immunocytochemistry: (5 μg/ml) Immunohistochemistry: (5 μg/ml) See IHC2011-6 for prediluted and detailed paraffin protocols.
Optimal working dilutions must be determined by end user.
Immunohysto/cyto chemistry Protocols
Ideal specimens are obtained from frozen sections from shock-frozen tissue samples. The frozen sections are dried in the air and then fixed with acetone at -20°C for 10 min. Excess acetone is allowed to evaporate at 15-25°C. Material fixed in alcohol and embedded in paraffin can also be used (2). Formaldehyde fixation will reduce or eliminate the intensity of staining depending on the conditions under which it is performed. Other fixation conditions must be first tested by the investigator.
It is advantageous to block unspecific binding sites by overlaying the sections with fetal calf serum for 20-30 min at 15-25°C. Excess of fetal calf serum is removed by decanting before application of the antibody solution.
Cytocentrifuge preparations of single cells or cell smears are also fixed in acetone. These preparations should, however not be dried in the air. Instead, the excess acetone is removed by briefly washing in phosphate-buffered saline (PBS).
Further treatment is then as follows:
• Overlay the preparation with 10-20 μl antibody solution and incubate in a humid chamber at 37°C for 1 h.
• Dip the slide briefly in PBS and then wash 3 x in PBS for 3 min (using a fresh PBS bath in each case).
• Wipe the margins of the preparation dry and overlay the preparation with 10-20 μl of a solution of anti-mouse Ig-FITC or anti-mouse IgG-peroxidase solution and allow to incubate for 1 h at 37°C in a humid chamber.
• Wash the slide as described above.
The preparation must not be allowed to dry out during any of the steps.
If using an indirect immunofluorescence technique, the preparation should be overlaid with a suitable embedding medium (e.g. Moviol, Hoechst) and examined under the fluorescence microscope. If a POD-conjugate has been used as the secondary antibody, the preparation should be overlaid with a substrate solution (see below) and incubated at 15-25°C until a clearly visible redbrown color develops. A negative control (e.g. only the secondary antibody) should remain unchanged in color during this incubation period. Subsequently, the substrate is washed off with PBS and the preparation is stained, if desired, with hemalum stain for about 1 min. The hemalum solution is washed off with PBS; the preparation is embedded and examined.
Substrate solutions:
Aminoethyl-carbazole: Dissolve 2 mg 3-amino-9-ethylcarbazole with 1.2 ml dimethylsulfoxide and add 28.8 ml 50 mM Tris-HCI, pH 7.3, and 20 μl 3% H 2 O 2 (w/v). Prepare solution freshly each day. Diaminobenzidine: Dissolve 25 mg 3,3'-diaminobenzidine with 50 ml 50 mM Tris-HCI, pH 7.3, and add 40 μl 3% H 2 O 2 (w/v). Prepare solution freshly each day.
Biological Information
Immunogen
Purified desmin.
Clone
DE-B-5
Concentration
1 mg/mL
Host
Mouse
Specificity
The antibody reacts with desmin from human, pig, rat and taod. In tissue sections this antibody is used to stain skeletal, cardiac, visceral, and some vascular smooth muscle cells. Cell lines such as RD (ATCC CCL 136) and hamster BHK-21 are positive (Debus et al., 1983).
This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies.
FUNCTION: SwissProt: P17661 # Desmin are class-III intermediate filaments found in muscle cells. In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures. SIZE: 470 amino acids; 53536 Da SUBUNIT: Homopolymer. SUBCELLULAR LOCATION: Cytoplasm. DISEASE: SwissProt: P17661 # Defects in DES are the cause of desmin-related cardio- skeletal myopathy (CSM) [MIM:601419]; also known as desmin-related myopathy (DRM). CSM is characterized by skeletal muscle weakness associated with cardiac conduction blocks, arrhythmias, restrictive heart failure, and by intracytoplasmic accumulation of desmin-reactive deposits in cardiac and skeletal muscle cells. A desmin-related myopathy can have a distal onset, it is then known as hereditary distal myopathy (HDM). & Defects in DES are the cause of dilated cardiomyopathy 1I (CMD1I) [MIM:604765]. CMD1I is an autosomal form of dilated cardiomyopathy characterized by ventricular dilatation and impaired systolic function. SIMILARITY: SwissProt: P17661 ## Belongs to the intermediate filament family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain antibody refrigerated at 2-8°C in undiluted aliquots for up to 6 months. DO NOT FREEZE.
