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ABE2901-25UL Anti-Dcp2

Anti-Dcp2, Cat. No. ABE2901, is a highly specific rabbit polyclonal antibody that targets m7GpppN-mRNA hydrolase / NUDT20 (Dcp2) and has been tested for use in Western Blotting.

Anti-Dcp2: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Epigenetics & Nuclear Function Gene Symbol: DCP2 UniProt Number: Q8IU60
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ABE2901-25UL
25 µL  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HWBRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABE2901-25UL
      DescriptionAnti-Dcp2
      Alternate Names
      • m7GpppN-mRNA hydrolase
      • EC:3.6.1.62
      • Nucleoside diphosphate-linked moiety X motif 20
      • Nudix motif 20
      • mRNA-decapping enzyme 2
      • hDpc
      Background Informationm7GpppN-mRNA hydrolase (UniProt: Q8IU60; also known as EC:3.6.1.62, Nucleoside diphosphate-linked moiety X motif 20, Nudix motif 20, mRNA-decapping enzyme 2, hDpc) is encoded by the DCP2 (also known as NUDT20) gene (Gene ID: 167227) in human. Dcp2 is a manganese-dependent decapping metalloenzyme that catalyzes the cleavage of the cap structure on mRNAs. It removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Dcp2 contains a functional Nudix (nucleotide diphosphate linked to an X moiety) motif (aa 95-226) that is essential for its decapping activity. Two isoforms of Dcp2 have been described that are produced by alternative splicing. Dcp2 is reported to play a role in replication-dependent histone mRNA degradation. It has higher activity towards mRNAs that lack a poly(A) tail and does not display any activity towards a cap structure lacking an RNA moiety. Dcp2 is predominantly cytoplasmic, however, a small amount may also be found in the nucleus. Higher expression of Dcp2 has been reported in subjects with celiac disease with higher levels in the nuclear fraction. (Ref.: Wang, Z., et al. (2002). Proc. Natl. Acad. Sci. USA 99(20); 12663-12668; Castellanos-Rubio, A., et al. (2016). Science 352(6281); 91-95).
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal antibody in serum with 0.05% sodium azide.
      Applications
      ApplicationAnti-Dcp2, Cat. No. ABE2901, is a highly specific rabbit polyclonal antibody that targets m7GpppN-mRNA hydrolase / NUDT20 (Dcp2) and has been tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Dcp2 in 293T KO, K562, 293T and MEF DCP 2 lysates (Courtesy of Dr. Mike Kiledjian at Rutgers University).

      Western Blotting Analysis: A representative lot detected Dcp2 in Western Blotting applications (Castellanos-Rubio, A., et. al. (2016). Science. 352(6281):91-5; Li, Y., et. al. (2012). Mol Cell Biol. 32(6):1164-72; Jiao, X., et. al. (2006). Mol Cell. 24(5):713-22; Li, Y., et. al. (2008). Mol Cell Biol. 28(3):939-48; Song, M.G., et. al. (2010). Mol Cell. 40(3):423-32; Li, Y., et. al. (2012). Mol Cell Biol. 32(6):1164-72).
      Biological Information
      ImmunogenHis-tagged full length recombinant human m7GpppN-mRNA hydrolase (Dcp2).
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects m7GpppN-mRNA hydrolase (Dcp2) in human and murine cells.
      IsotypeIgG
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • DCP2
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~51 kDa observed; 48.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in 293T control KO cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Dcp2 (NUDT20) in lysate from 293T control knockout cells, but did not react with Dcp2 knockout 293T cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      ABE2901-25UL 04054839460043