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MABT902 Anti-Connexin 43 Antibody, clone P2C4

Anti-Connexin 43 Antibody, clone P2C4, Cat. No. MABT902, is a mouse monoclonal antibody that targets Connexin-43 and is tested for use in Electron Microscopy, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Anti-Connexin 43 Antibody, clone P2C4: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Cell Structure Gene Symbol: Gja1, Cxn-43 UniProt Number: P08050
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MABT902
100 μg  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, M, HWB, IH(P), EM, ICC, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT902
      DescriptionAnti-Connexin 43 Antibody, clone P2C4
      Alternate Names
      • Gap junction alpha-1 protein
      • CX43
      • Gap junction 43 kDa heart protein
      Background InformationGap junction alpha-1 protein (UniProt: P08050; also known as Connexin-43, CX43, Gap junction 43 kDa heart protein) is encoded by the Gja1 (also known as Cxn-43) gene (Gene ID: 24392) in rat. The gap junction channels are dodecamers of transmembrane proteins of the connexin family. Connexins usually have a short half-life ( 1.5 5 h) and this short half-life is a key element of coupling regulation as it allows very dynamic and acute changes in gap junction regulation and gap junctional communication. In cardiomyocytes three types of connexins have been identified: Connexin 40, 43, and 45. Each of these connexins form channels with unique and specific electrophysiological properties. Connexin-43 is a multi-pass membrane protein and is the most ubiquitous connexin with widespread tissue expression pattern. Connexin-43 can be phosphorylated at multiple sites and phosphorylation is shown to regulate the kinetics of its trafficking, assembly, gating and turnover in a cell-cycle-stage specific manner. Phosphorylation at Ser325, Ser328 and Ser330 by casein kinase I is reported to modulate gap junction assembly while phosphorylation of Ser368 by protein kinase C gamma isoform leads to disassembly of gap junction plaques and inhibition of gap junction activity. During myocardial ischemia connexin-43 is shown to become dephosphorylated that allows gap junctions to be opened and convey the propagation of ischemia injury. In bladder smooth muscle cells, connexin-43 displays a circadian cycle with low levels during the sleep phase.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Connexin 43 Antibody, clone P2C4, Cat. No. MABT902, is a mouse monoclonal antibody that targets Connexin-43 and is tested for use in Electron Microscopy, Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      • Electron Microscopy
      • Immunocytochemistry
      • Immunoprecipitation
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Connexin 43 in rat heart tissue.

      Immunoprecipitation Analysis: A representative lot detected Connexin 43 in Immunoprecipitation applications (Solan, J.L., et. al. (2003). J Cell Sci. 116(Pt 11):2203-11).

      Electron Microscopy Analysis: A representative lot detected Connexin 43 in Electron Microscopy applications (Sosinsky, G.E., et. al. (2007). Biochem J. 408(3):375-85).

      Immunocytochemistry Analysis: A representative lot detected Connexin 43 in Immunocytochemistry applications (Sosinsky, G.E., et. al. (2007). Biochem J. 408(3):375-85).

      Western Blotting Analysis: A representative lot detected Connexin 43 in Western Blotting applications (Sosinsky, G.E., et. al. (2007). Biochem J. 408(3):375-85).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 23 amino acids from the C-terminal region of rat connexin 43.
      CloneP2C4
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone P2C4 detects Connexin 43 in human heart. It targets an epitope within 23 amino acids from the C-terminal region. It primarily binds to the non-phosphorylated isoform around Ser364/365.
      IsotypeIgG2aκ
      Species Reactivity
      • Rat
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse, Rat. Predicted to react with Hamster, Canine, Rabbit, Bovine, Porcine, Guinea Pig, Rhesus Macaque based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Gja1
      • Cxn-43
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight43.03 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in human heart tissue.

      Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected Connexin 43 in human heart tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABT902 04054839204081