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MABT528 Anti-Connexin 43 Antibody, CT Antibody, clone 1C5.1

Anti-Connexin 43, CT, clone 1C5.1, Cat. No. MABT528 is a mouse monoclonal antibody validated for use in Immunohistochemistry (Paraffin) and Western Blotting for the detection of connexin 43.

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Research Category: Cell Structure Gene Symbol: GJA1, AVSD3, CMDR, GJAL, HSS, ODDD UniProt Number: P17302
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MABT528
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, HIH(P), WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT528
      ReplacesMAB3068
      DescriptionAnti-Connexin 43 Antibody, CT Antibody, clone 1C5.1
      Alternate Names
      • Gap junction alpha-1 protein
      • Connexin-43
      • Cx43
      • Gap junction 43 kDa heart protein
      Background InformationGap junction alpha-1 protein (UniProt P17302; also known as Connexin-43, Cx43, Gap junction 43 kDa heart protein) is encoded by the GJA1 (also known as AVSD3, CMDR, GJAL, HSS, ODDD) gene (Gene ID 2697) in human. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexins, through which materials of low molecular weight diffuse from one cell to a neighboring cell. Connexin-43 is a polytopic integral membrane protein oligomerizes into a hexamer or connexon. It is he most ubiquitously expressed connexin and contains a long C-terminal tail, an N-terminal domain, and multiple transmembrane domains. The C-terminal tail domain of human connexin-43 contains 50 amino acids and includes post-translational modification sites, as well as binding sites for transcription factors, cytoskeleton elements, and other proteins. The C-terminal tail regulates pH gating and channel assembly, whereas the N-terminal domain is involved in channel gating and oligomerization and controls the switch between channel open and closed states. Mutations in connexin-43 result in multiple disorders, such as oculodentodigital dysplasia that is characterized by microcornea, microphthalmia, and syndactyly of the fourth and fifth fingers. Connexin-43 is shown to negatively regulate growth of colon cancer cells, in part by enhancing apoptosis. Loss of connexin-43 expression in colorectal cancers is correlated with significantly shorter relapse-free and overall survival.

      Ref.:
      Sirnes, S et al. (2012). Int. J. Cancer 131, 570-581.
      Naus, CC et al. (2010). Nat. Rev. Cancer. 10, 435-441.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Connexin 43, CT, clone 1C5.1, Cat. No. MABT528 is a mouse monoclonal antibody validated for use in Immunohistochemistry (Paraffin) and Western Blotting for the detection of connexin 43.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected cconnexin 43 in human cerebellum, heart muscle, and kidney tissue sections.
      Biological Information
      ImmunogenGST-tagged recombinant human connexin 43 C-terminal cytoplasmic tail fragment.
      Clone1C5.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 1C5.1 targets an epitope within the second half of the C-terminal cytoplasmic tail.
      IsotypeIgG1κ
      Species Reactivity
      • Rat
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GJA1
      • AVSD3
      • CMDR
      • GJAL
      • HSS
      • ODDD
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~43 kDa observed. 42.88/42.90 kDa (human/rat) calculated (Met1 removed). Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HUVEC lysate.

      Western Blotting Analysis: 10 ng/mL of this antibody detected connexin 43 in 10 µg of HUVEC lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABT528 04054839114465