Millipore Sigma Vibrant Logo

04-135 Anti-Brachyury Antibody, clone 3E4.2

View Products on Sigmaaldrich.com
04-135
100 µg  
Preis nicht abrufbar
Die Mindestmenge muss ein Vielfaches sein von
Maximum Quantity is
Bei Bestätigung Weitere Informationen
Sie haben () gespart
 
Bitte erfragen
Eingeschränkte Verfügbarkeit
Eingeschränkte Verfügbarkeit
Lieferbar 
Produkt wurde eingestellt
Begrenzter Lagerbestand
Bestätigung der Verfügbarkeit erforderlich
    Restmenge: Angebot folgt
      Restmenge: Angebot folgt
      Bitte erfragen
      Kontakt zum Kundenservice
      Contact Customer Service

      Sonderaktionen

       

      Kontakt zum Kundenservice

      Übersicht

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-135
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Brachyury Antibody, clone 3E4.2
      Alternate Names
      • T brachyury (mouse) homolog
      • T brachyury-like
      • T protein
      • T
      • brachyury homolog (mouse)
      • transcription factor T
      Background InformationThe Brachyury protein is a T-box transcription factor that is one of the earliest indicators of mesoderm formation during embryonic development. As the brachyury protein family is essential for the formation of the posterior body in all vertebrates, Brachyury has been shown to be expressed during gastrula and neurula stages, as well as in the developing notochord. Consequently, when mutations occur within the brachyury gene, severe vertebral malformations have been found to occur, as a direct result. In addition, current findings also indicate that the dysregulation of brachyury may also be linked to the formation of chordomas, which are malignant tumors that develop along the spine.
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ in buffer containing PBS with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Brachyury Antibody, clone 3E4.2 is validated for use in WB for the detection of Brachyury.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenSynthetic human brachyury peptide, a.a. 224-435.
      EpitopeC-terminus
      Clone3E4.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis monoclonal antibody recognizes the brachyury protein.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteReacts with human. Reactivity with other species has not been tested.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site. It binds through a region in its N-terminus, called the T-box, and effects transcription of genes required for mesoderm formation and differentiation. The protein is localized to notochord-derived cells.
      Gene Symbol
      • MGC104817
      • OTTHUMP00000017588
      • TF
      • TFT
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O15178 # Involved in the transcriptional regulation of genes required for mesoderm formation and differentiation. Binds to a palindromic site (called T site) and activates gene transcription when bound to such a site.
      SIZE: 435 amino acids; 47443 Da
      SUBUNIT: Monomer (By similarity).
      Nucleus.
      DISEASE: SwissProt: O15178 # Genetic variations in T are associated with susceptibility to neural tube defects (NTD) [MIM:182940]. NTD are common congenital malformations. Spina bifida, which results from malformations in the caudal region of the neural tube, is compatible with life but associated with significant morbidity, including lower limb paralysis.
      SIMILARITY: SwissProt: O15178 ## Contains 1 T-box DNA-binding domain.
      Stem Cell Type
      • Mesenchymal Stem Cells
      Molecular Weight47 kDa
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western blot on NT2 cell lysates.

      Western Blotting:
      Recommended working dilutions are 1:1000.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. Avoid repeat freeze/thaw cycles.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Bestellnummer GTIN
      04-135 04053252323157