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05-449 Anti-Bin1 Antibody, clone 99D

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05-449
200 µg  
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      Übersicht

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RIP, WB, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number05-449
      ReplacesMAB4131
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Bin1 Antibody, clone 99D
      Product Information
      FormatPurified
      Presentation0.1M Tris-glycine, pH 7.4, containing 0.05% sodium azide
      Quality LevelMQ100
      Applications
      ApplicationAnti-Bin1 Antibody, clone 99D is an antibody against Bin1 for use in IP, WB, IH.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry
      Biological Information
      ImmunogenPartial fusion protein corresponding to amino acids 189-398 of human Bin1. Clone 99D
      Cloneclone 99D
      HostMouse
      SpecificityBin1
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes several isoforms of a nucleocytoplasmic adaptor protein, one of which was initially identified as a MYC-interacting protein with features of a tumor suppressor. Isoforms that are expressed in the central nervous system may be involved in synaptic vesicle endocytosis and may interact with dynanim, synaptojanin, endophilin, and clathrin. Isoforms that are expressed in muscle and ubiquitously expressed isoforms localize to the cytoplasm and nucleus and activate a caspase-independent apoptotic process. Studies in mouse suggest that this gene plays an important role in cardiac muscle development. Alternate splicing of the gene results in ten transcript variants encoding different isoforms. Aberrant splice variants expressed in tumor cell lines have also been described.
      Gene Symbol
      • BIN1
      • AMPH2
      • amphiphysin-like
      • MGC10367
      • DKFZp547F068
      • AMPHL
      • SH3P9
      Purification MethodProtein G Chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O00499 # May be involved in regulation of synaptic vesicle endocytosis. May act as a tumor suppressor and inhibits malignant cell transformation.
      SIZE: 593 amino acids; 64699 Da
      SUBUNIT: Heterodimer with AMPH. Binds SH3GLB1 (By similarity). Binds to SYNJ1 and DNM1 through its SH3 domain, and to clathrin through a region outside of the SH3 domain. Also binds AP2A2. Interacts with the N-terminal transactivation domain of MYC in a manner requiring the integrity of the conserved MYC box regions 1 and 2. Interacts with BIN2. Interacts with HCV NS5A through its SH3 domain.
      SUBCELLULAR LOCATION: Isoform BIN1: Nucleus. & Isoform IIA: Cytoplasm.
      TISSUE SPECIFICITY: Ubiquitous. Highest expression in the brain and muscle. Isoform IIA is expressed only in the brain where it is concentrated in axon initial segments and nodes of Ranvier. Isoform BIN1 is widely expressed with highest expression in skeletal muscle.
      PTM: Phosphorylated by protein kinase C (By similarity).
      DISEASE: "SwissProt: O00499 # Defects in BIN1 are the cause of centronuclear myopathy autosomal recessive (ARCNM) [MIM:255200]; also known as autosomal recessive myotubular myopathy. Centronuclear myopathies are congenital muscle disorders characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers."
      SIMILARITY: SwissProt: O00499 ## Contains 1 BAR domain. & Contains 1 SH3 domain.
      Molecular Weight56kDa
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysate of C2C12 cells.
      Sales RestrictionsDue to license agreement restrictions, this product cannot be purchased for resale.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -20°C
      Packaging Information
      Material Size200 µg
      Global Trade Item Number
      Bestellnummer GTIN
      05-449 04053252282911