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MABT1508-25UG Anti-Alpha-Catenin Antibody, clone 1G5

Anti-Alpha-Catenin, clone 1G5, Cat. No. MABT1508, is a mouse monoclonal antibody that detects Catenin alpha 1 and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Anti-Alpha-Catenin Antibody, clone 1G5: SDB (Sicherheitsdatenblätter), Analysenzertifikate und Qualitätszertifikate, Dossiers, Broschüren und andere verfügbare Dokumente.
Research Category: Cell Structure Gene Symbol: CTNNA1 UniProt Number: E1C7M7
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MABT1508-25UG
25 μg  
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      Übersicht

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ch, HICC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT1508-25UG
      DescriptionAnti-Alpha-Catenin Antibody, clone 1G5
      Alternate Names
      • Catenin alpha 1
      Background InformationCatenin alpha 1 (UniProt: E1C7M7) is encoded by the CTNNA1 gene in chicken. Catenins are a large family of cell-cell adhesion molecules that are involved in development and in epithelial cell polarity. Catenins are reported to associate with both E- and N-cadherins. The association of catenins to cadherins creates a complex that is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Alpha-Catenin is considered as an important regulator of actin dynamics in cell-cell adhesion and it forms a heterodimer with beta-Catenin and links the cadherins to actin. Unlike other catenins, alpha-catenin does not bind to cadherins directly, but is linked to the adhesive complexes of AJs via association with -catenin. Alpha-catenin can also bind and bundle actin filaments and can interact with a number of protein regulators of actin filament turnover. Usually cells contain a significant amount of cadherin-free alpha-catenin and this pool of free alpha-catenin can impact actin dynamics, such as limiting Arp2/3-based actin polymerization and severing by cofilin that ultimately promotes the assembly and stabilization of unbranched filament bundles. Alpha-catenin expression in a colon cancer cell lines has been correlated with increased Tcf-dependent transcription and is shown to inhibit beta-catenin signaling in the nucleus by interfering with the formation of a beta-catenin-Tcf-DNA complex. (Ref.: Giannini, AL et al. (2000). J. Bol. Chem. 275(29); 21883-8).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Alpha-Catenin, clone 1G5, Cat. No. MABT1508, is a mouse monoclonal antibody that detects Catenin alpha 1 and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated Alpha-Catenin in Immunoprecipitation applications (Knudsen, K.A., et. al. (1995). J Cell Biol. 130(1):67-77; Johnson, K.R., et. al. (1993). Exp Cell Res. 207(2):252-60).

      Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected Alpha-Catenin in A431 cell line.

      Western Blotting Analysis: A representative lot detected Alpha-Catenin in Western Blotting applications (Nieman, M.T., et. al. (1999). J Cell Sci. 112 ( Pt 10):1621-32; Knudsen, K.A., et. al. (1995). J Cell Biol. 130(1):67-77; Johnson, K.R., et. al. (1993). Exp Cell Res. 207(2):252-60).

      Immunocytochemistry Analysis: A representative lot detected Alpha-Catenin in Immunocytochemistry applications (Johnson, K.R., et. al. (1993). Exp Cell Res. 207(2):252-60).
      Biological Information
      ImmunogenMBP-conjugated full-length chicken recombinant Catenin alpha 1.
      Clone1G5
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 1G5 specifically detects alpha-catenin 1 in human and chicken cells.
      IsotypeIgG1κ
      Species Reactivity
      • Chicken
      • Human
      Species Reactivity NoteChicken, Human.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • CTNNA1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~108 kDa observed; 59.26 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Alpha-Catenin in A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABT1508-25UG 04054839540295