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MABF2086-25UG Anti-Adenylate Cyclase Toxin Antibody, B. pertussis Antibody, clone 3D1

Anti-Adenylate Cyclase Toxin, B. pertussis, clone 3D1 , Cat. No. MABF2086, is a mouse monoclonal antibody that detects Adenylate cyclase (AC) toxin from Bordetella pertussis and has been tested for use in ELISA, Flow Cytometry, Inhibition Assay, Neutralizing, and Western Blotting.

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MABF2086-25UG
25 μg  
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      Übersicht

      Replacement Information
      Description
      Catalogue NumberMABF2086-25UG
      DescriptionAnti-Adenylate Cyclase Toxin Antibody, B. pertussis Antibody, clone 3D1
      Alternate Names
      • Adenylate cyclase toxin
      • Bordetella pertussis Toxin
      • ACT
      • CyaA
      Background InformationAdenylate Cyclase Toxin (UniProt: C8C508; also known as ACT or CyaA) is one of several virulence factors produced by the bacterium Bordetella pertussis. Bordetella pertussis, a gram-negative bacterium, is shown to be the causative agent for whooping cough. It produces several virulence factors, including Cya A. The CyaA toxin is a bifunctional protein of 1,706 residues consisting of an NH2-terminal catalytic domain of 400 amino acids and a COOH-terminal part with 1,306 residues. The CyaA polypeptide is synthesized as an inactive protoxin, which is then converted to an active toxin by posttranslational palmitoylation of two internal lysine residues (Lys 856 and 963). Upon interaction of its C-terminal hemolysin moiety with the cell surface receptor alphaM-beta2 integrin ((Cd11b/Cd18), the N-terminal cyclase domain translocates into the host cell cytosol where it generates supraphysiological levels of cAMP concentration, which inhibits host cell anti-bacterial activities and apoptotic death of macrophages. CyaA toxin is also shown to elicit potassium ion efflux from erythrocytes in a process that is thought to be a precursor event to osmotic lysis of erythrocytes.Clone 3D1 is shown to cause both an enhancement of hemolysis and inhibition of intoxication in erythrocytes. It also inhibits toxin-induced cAMP accumulation in Jurkat cells. (Ref.: Guermonprez, P., et al. (2001). J. Exp. Med. 193(9); 1035 1044; Gray, MC., et al. (2001). J. Bacteriol. 183(20); 5904-5910; Holubova, J., et al. (2012). Infect. Immun. 80(3); 1181 1192).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without preservatives.
      Applications
      ApplicationAnti-Adenylate Cyclase Toxin, B. pertussis, clone 3D1 , Cat. No. MABF2086, is a mouse monoclonal antibody that detects Adenylate cyclase (AC) toxin from Bordetella pertussis and has been tested for use in ELISA, Flow Cytometry, Inhibition Assay, Neutralizing, and Western Blotting.
      Key Applications
      • ELISA
      • Flow Cytometry
      • Inhibition
      • Neutralizing
      • Western Blotting
      Application NotesInhibition Analysis: A representative lot inhibited AC toxin-induced cAMP accumulation in Jurkat cells and inhibited intoxication of sheep erythrocytes. (Bumba, L., et. al. (2016). Mol Cell. 62(1):47-62; Gray, M.C., et. al. (2001). J Bacteriol. 183(20):5904-10; Holubova, J., et. al. (2012). Infect Immun. 80(3):1181-92; Hewlett, E.L., et. al. (2006). Mol Microbiol. 59(2):447-59).

      Flow Cytometry Analysis: A representative lot detected Adenylate Cyclase Toxin, B. pertussis in Flow Cytometry applications (Gray, M.C., et. al. (2001). J Bacteriol. 183(20):5904-10).

      Western Blotting Analysis: A representative lot detected Adenylate Cyclase Toxin, B. pertussis in Western Blotting applications (Lee, S.J., et. al. (1999). Infect Immun. 67(5):2090-5).

      ELISA Analysis: A representative lot detected Adenylate Cyclase Toxin, B. pertussis in ELISA applications (Weingart, C.L., et. al. (2000). Infect Immun. 68(12):7152-5; Wang, X., et. al. (2015). J Biol Chem. 290(38):23025).

      Neutralizing Analysis: A representative lot neutralized Adenylate Cyclase Toxin action. (Holubova, J., et. al. (2012). Infect Immun. 80(3):1181-92.
      Biological Information
      ImmunogenAdenylate cyclase (AC) toxin from Bordetella pertussis.
      Clone3D1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 3D1 is a mouse monoclonal antibody that detects Adenylate cyclase (AC) toxin from Bordetella pertussis.
      IsotypeIgG1κ
      Species Reactivity
      • Bacteria
      Species Reactivity NoteBacteria.
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G purified
      Molecular Weight~220 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Adenylate cyclase toxin from Bordetella pertussis.

      Western Blotting Analysis: 0.2 µg/mL of this antibody detected Adenylate Cyclase Toxin from Bordetella pertussis.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Bestellnummer GTIN
      MABF2086-25UG 04054839499661

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