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Die folgenden MAPmates™ sollten nicht zusammen analysiert werden: -MAPmates™, die einen unterschiedlichen Assaypuffer erfordern. -Phosphospezifische und MAPmate™ Gesamtkombinationen wie Gesamt-GSK3β und Gesamt-GSK3β (Ser 9). -PanTyr und locusspezifische MAPmates™, z.B. Phospho-EGF-Rezeptor und Phospho-STAT1 (Tyr701). -Mehr als 1 Phospho-MAPmate™ für ein einziges Target (Akt, STAT3). -GAPDH und β-Tubulin können nicht mit Kits oder MAPmates™, die panTyr enthalten, analysiert werden.
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48-602MAG
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Anti-Adeno-associated virus 9, clone HL2370, Cat. No. MABF2325, is a mouse monoclonal antibody that detects Adeno-associated viruses serotype 9 capsid protein. and has been tested for use in Dot Blot, ELISA, and Neutralizing Applications.
More>>Anti-Adeno-associated virus 9, clone HL2370, Cat. No. MABF2325, is a mouse monoclonal antibody that detects Adeno-associated viruses serotype 9 capsid protein. and has been tested for use in Dot Blot, ELISA, and Neutralizing Applications. Less<<
Adeno-associated virus (AAV) is a small (25-nm), non-enveloped virus that packages a linear single-stranded DNA genome. It belongs to the family Parvoviridae and productive infection by AAV occurs only in the presence of a helper virus, which could either be adenovirus or herpesvirus. After successful infection, two stages of AAV life cycle have been described - a lytic stage and a lysogenic stage. In the presence of helper virus, the lytic stage ensues and during this period, AAV undergoes productive infection with genome replication, viral gene expression, and virion production. The lysogenic stage is established in host cells in the absence of a helper virus and AAV integrates into the host genome at specific site, AAVS1 on human chromosome19. This site is favored due to the presence of a Rep binding element. AAV genome consists of two open reading frames, Rep and Cap, flanked by two 145 base inverted terminal repeats (ITR). The ITR base pairs allow for synthesis of the complementary DNA strand. Rep and Cap are translated to produce multiple distinct proteins. The Rep gene encodes Rep78, Rep68, Rep52, Rep40 and Cap gene produces VP1, VP2, VP3 capsid proteins. The Rep78 and Rep68 proteins participate in DNA replication process via their interaction with Rep-binding element (RBE) and terminal resolution site sequences located within the ITR. The Rep52 and Rep40 proteins are involved in the generation and accumulation of single-stranded viral genomes from double-stranded replicative intermediates. Thus far eleven serotypes of AAV have been identified, which differ in their tropism or the type of cells they infect. AAVs have been used as gene delivery vectors and recombinant AAV9 has been shown to cross blood-brain barrier in neonatal and adult mice and infect astrocytes through its interaction with astrocytic perivascular endfeet in direct contact with vascular endothelial cells. Clone HL2370, generated against AAV9 capsid, specifically binds only to AAV9 and is shown to neutralize infection in cells. (Ref.: Russell, WC et al. (2009). J. Gen. Virol. 90(1); 1-20; Tseng, Y-S., et al. (2016). J. Virol. Methods. 236; 105-110).
References
Product Information
Format
Purified
Presentation
Purified mouse monoclonal antibody IgG2a in PBS without preservatives.
Applications
Application
Anti-Adeno-associated virus 9, clone HL2370, Cat. No. MABF2325, is a mouse monoclonal antibody that detects Adeno-associated viruses serotype 9 capsid protein. and has been tested for use in Dot Blot, ELISA, and Neutralizing Applications.
Key Applications
Dot Blot
ELISA
Neutralizing
Application Notes
Dot Blot Analysis: A representative lot detected Adeno-associated virus 9 in Dot Blot applicaitons (Tseng, Y.S., et. al. (2016). J Virol Methods. 236:105-110).
Neutralizing Analysis: A representative lot neutralized Adenovirus infection in cells in Neutrailizing applicaitons (Tseng, Y.S., et. al. (2016). J Virol Methods. 236:105-110).
ELISA Analysis: A representative lot detected Adeno-associated virus 9 in ELISA applicaitons (Tseng, Y.S., et. al. (2016). J Virol Methods. 236:105-110).
Clone HL2370 is a mouse monoclonal antibody that specifically detects AAV9 capsid protein.
Isotype
IgG2aκ
Species Reactivity
Virus
Species Reactivity Note
Virus (Adeno virus)
Antibody Type
Monoclonal Antibody
Purification Method
Protein G purified
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assurance
Evaluated by Dot Blot with AAV8 and AAV9 virus like particles.
Dot Blot Analysis: Various dilutions of AAV8 and AAV9 virus like particles were probed with Adenovirus 9. This antibody specifically detected AAV9, but not AAV8.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Adeno-associated viruses (AAVs) are promising viral vectors for therapeutic gene delivery, and the approval of an AAV1 vector for the treatment of lipoprotein lipase deficiency has heralded a new and exciting era for this system. However, preclinical and clinical studies show that neutralization from pre-existing antibodies is detrimental for medical application and this hurdle must be overcome before full clinical realization can be achieved. Thus the binding sites for capsid antibodies must be identified and eliminated through capsid engineering. Towards this goal and to recapitulate patient polyclonal responses, a panel of six new mouse monoclonal antibodies (MAbs) has been generated against AAV8 and AAV9 capsids, two vectors being developed for therapeutic application. Native (capsid) dot blot assays confirmed the specificity of these antibodies for their parental serotypes, with the exception of one MAb, HL2372, selected to cross-react against both capsids. Furthermore, in vitro assays showed that these MAbs are capable of neutralizing virus infection. These MAbs will be utilized for structural mapping of antigenic footprints on their respective capsids to inform development of the next generation of rAAV vectors capable of evading antibody neutralization while retaining parental tropism.
